1. Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1-dependent mechanism
- Author
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Per-Olof Hasselgren, Aniket Gurav, Estibaliz Castillero, Steven Tizio, Nima Alamdari, Zaira Aversa, and Victoria Petkova
- Subjects
endocrine system diseases ,Ubiquitin-Protein Ligases ,Muscle Fibers, Skeletal ,Biophysics ,Muscle Proteins ,Nerve Tissue Proteins ,FOXO1 ,Resveratrol ,Protein degradation ,Biochemistry ,Dexamethasone ,Article ,Cell Line ,Tripartite Motif Proteins ,chemistry.chemical_compound ,Atrophy ,Sirtuin 1 ,Ubiquitin ,Stilbenes ,medicine ,Animals ,skin and connective tissue diseases ,Molecular Biology ,SKP Cullin F-Box Protein Ligases ,biology ,Myogenesis ,organic chemicals ,food and beverages ,Acetylation ,Forkhead Transcription Factors ,Cell Biology ,medicine.disease ,Molecular biology ,Muscle atrophy ,Rats ,Cell biology ,chemistry ,biology.protein ,medicine.symptom ,hormones, hormone substitutes, and hormone antagonists - Abstract
Resveratrol (3,5,4’-trihydroxystilbene) has been ascribed multiple beneficial biological effects but the influence of resveratrol on glucocorticoid-induced muscle atrophy is not known. We examined the effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression, FOXO1 acetylation, protein degradation and atrophy in cultured L6 myotubes. In addition, the role of the deacetylase SIRT1 in the effects of resveratrol was determined by transfecting myotubes with SIRT1 siRNA. The catabolic effects of dexamethasone were prevented by resveratrol and the protective effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression were abolished in myotubes transfected with SIRT1 siRNA. Results suggest that resveratrol can prevent glucocorticoid-induced muscle wasting and that this effect is at least in part SIRT1-dependent.
- Published
- 2012
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