1. Genetic mapping of tomato cDNA clones encoding the chloroplastic and the cytosolic isozymes of superoxide dismutase
- Author
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Nurit Magal, Daniel Zamir, Mohamad Abu-Abied, Esra Galun, and Rafael Perl-Treves
- Subjects
clone (Java method) ,Chloroplasts ,biology ,Superoxide Dismutase ,Chromosome Mapping ,food and beverages ,DNA ,General Medicine ,Plants ,biology.organism_classification ,Biochemistry ,Genetic analysis ,Molecular biology ,Isozyme ,Lycopersicon ,Isoenzymes ,Superoxide dismutase ,Cytosol ,Gene mapping ,Complementary DNA ,Genetics ,biology.protein ,Restriction fragment length polymorphism ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Abstract
The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between marker TG46 and TG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 between TG24 and TG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis.
- Published
- 1990
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