1. Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse.
- Author
-
Lee CY and Pegoraro B
- Subjects
- Animals, Electrophoresis, Polyacrylamide Gel, Epitopes, Genes, Isoelectric Focusing, Male, Mice, Mice, Inbred C3H genetics, Mice, Inbred C57BL genetics, Mice, Inbred DBA genetics, Phosphoglycerate Kinase immunology, Testis enzymology, Genetic Variation, Phosphoglycerate Kinase genetics
- Abstract
Three electrophoretic variants of 3-phosphoglycerate kinase 2 (PGK-2A,PGK-2B, and PGK-2C) were purified from DBA/2J, C3H/HeJ, and C57L/J mice, respectively. PGK-2C exhibits only 2% of the specific activity of PGK-2A and PGK-2B in the reaction leading to the formation of 1,3-diphosphoglycerate. Compared to PGK-2A and PGK-2B, PGK-2C exhibits broader coenzyme specificity and lower Kms for substrate and coenzymes. Incubation at 45C revealed immunionactivation and double immunodiffusion studies showed that mice carrying any one of these three PGK-2 alleles have similar amounts of proteins for PGK-1 and PGK-2 in testes. The results of these studies suggest that low PGK-2C activity in C57L/J mice is a result of a structural rather than a regulatory gene mutation.
- Published
- 1979
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