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Start Over Author "Kyrtopoulos, S. A." Journal biochemical journal
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3. Thiol-blocking reagents and phosphate acetyltransferase catalysis, and the assessment of protection by adsorbed molecules.

Author

Kyrtopoulos SA and Satchell DP

Subjects
Acetamides pharmacology, Acetyl Coenzyme A, Adsorption, Benzoates pharmacology, Catalysis, Iodoacetates pharmacology, Mercuribenzoates pharmacology, Sulfhydryl Reagents antagonists & inhibitors, Acetyltransferases antagonists & inhibitors, Sulfhydryl Reagents pharmacology
Abstract

Phosphate acetyltransferase (EC 2.3.1.8) is shown to be unusually sensitive to thiol-blocking reagents, and one or more thiol groups probably catalyse the surface reaction. A novel method for assessing protection by adsorbed species is described. The adsorbed substrate molecules acetyl-CoA and phosphate are found to provide substantial protection against thiol-blocking reagents.

Published
1974
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