1. The direct interaction of NME3 with Tip60 in DNA repair
- Author
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Zee-Fen Chang, Ning Tsao, Ya-Chi Yang, and Yu-Jyun Deng
- Subjects
0301 basic medicine ,Gene isoform ,DNA Repair ,DNA damage ,Kinase ,DNA repair ,DNA replication ,Cell Biology ,Histone acetyltransferase ,NM23 Nucleoside Diphosphate Kinases ,Biology ,Biochemistry ,Lysine Acetyltransferase 5 ,03 medical and health sciences ,030104 developmental biology ,Ribonucleotide reductase ,Ribonucleotide Reductases ,MCF-7 Cells ,biology.protein ,Humans ,Molecular Biology ,Function (biology) ,HeLa Cells ,Histone Acetyltransferases - Abstract
Cellular supply of dNTPs via RNR (ribonucleotide reductase) is crucial for DNA replication and repair. It has been shown that DNA-damage-site-specific recruitment of RNR is critical for DNA repair efficiency in quiescent cells. The catalytic function of RNR produces dNDPs. The subsequent step of dNTP formation requires the function of NDP kinase. There are ten isoforms of NDP kinase in human cells. In the present study, we identified NME3 as one specific NDP kinase that interacts directly with Tip60, a histone acetyltransferase, to form a complex with RNR. Our data reveal that NME3 recruitment to DNA damage sites depends on this interaction. Disruption of interaction of NME3 with Tip60 suppressed DNA repair in serum-deprived cells. Thus Tip60 interacts with RNR and NME3 to provide site-specific synthesis of dNTP for facilitating DNA repair in serum-deprived cells which contain low levels of dNTPs.
- Published
- 2016
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