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1. Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment...

Author

Mondragon, Angelo and Griffith, Eric C.

Subjects
*ESCHERICHIA coli
Abstract

Investigates the overexpression and purification of human calcineurin alpha from `Escherichia coli'. Assessment of the catalytic functions of residues surrounding the binuclear metal center; Importance of the binuclear metal center and its surrounding residues in catalysis by calcineurin; Performance of a systematic alanine-scan mutagenesis.

Published
1997
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