1. High-Resolution Crystal Structure of MltE, an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase from Escherichia coli
- Author
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Kathrin Meindl, Shahriar Mobashery, Isabel Usón, Cecilia Artola-Recolons, Leticia I. Llarrull, Malika Kumarasiri, Elena Lastochkin, César Carrasco-López, Iñaki M. de Ilarduya, Juan A. Hermoso, Artola-Recolons, Cecilia, Carrasco-Lopez, Cesar, Llarrull, Leticia I, Kumarasiri, Malika, Lastochkin, Elena, Martinez, De Ilarduya Inaki, Meindl, Kathrin, Uson, Isabel, Mobashery, Shahriar, and Hermoso, Juan A
- Subjects
Models, Molecular ,degradative activity ,peptidoglycans ,Protein Conformation ,outer membrane ,Biology ,Crystallography, X-Ray ,medicine.disease_cause ,Biochemistry ,Article ,Substrate Specificity ,Cell wall ,chemistry.chemical_compound ,Protein structure ,Catalytic Domain ,Escherichia coli ,medicine ,cell walls ,Escherichia coli Proteins ,Substrate (chemistry) ,high resolution crystal structure ,Lyase ,chemistry ,Lytic cycle ,Biocatalysis ,Peptidoglycan Glycosyltransferase ,Peptidoglycan ,escherichia coli ,inner leaflets ,Bacterial outer membrane ,Bacterial Outer Membrane Proteins - Abstract
El pdf del artículo es el manuscrito de autor (PMID:21341761)., The crystal structure of the first endolytic peptidoglycan lytic transglycosylase MltE from Escherichia coli is reported here. The degradative activity of this enzyme initiates the process of cell wall recycling, which is an integral event in the existence of bacteria. The structure sheds light on how MltE recognizes its substrate, the cell wall peptidoglycan. It also explains the ability of this endolytic enzyme to cleave in the middle of the peptidoglycan chains. Furthermore, the structure reveals how the enzyme is sequestered on the inner leaflet of the outer membrane. © 2011 American Chemical Society., Este trabajo fue finanaciado por las concesiones bfu2008-01711 y eu-cp223111.
- Published
- 2011
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