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Your search keyword '"Lukyanov, Konstantin"' showing total 5 results
Start Over Author "Lukyanov, Konstantin" Journal biochemistry
5 results on '"Lukyanov, Konstantin"'

2. The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore

Author

Mishin, Alexander S., Subach, Fedor V., Yampolsky, Ilia V., King, William, Lukyanov, Konstantin A., and Verkhusha, Vladislav V.

Subjects
Bacterial proteins -- Structure, Chromophores -- Structure, Flow cytometry -- Usage, Mutagenesis -- Analysis, Fluorescence -- Analysis, Biological sciences, Chemistry
Abstract

A new strategy of molecular evolution is used aimed at generating a red-emitting mutant green fluorescent protein (GFP) from a jelly fish Aequorea victoria. It is showed that the novel dual-color GFP mutant with green and red emission is suitable for multicolor flow cytometry as an additional color since it is separable from both green and red fluorescent tags.

Published
2008

3. Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcata

Author

Yampolsky, Ilia V., Remington S. James, Martynov, Valdimir I., Potapov, Victor K., Lukyanov, Sergey, and Lukyanov, Konstantin A.

Subjects
Chromophores -- Chemical properties, Chromophores -- Properties, Biological sciences, Chemistry
Abstract

A model compound for the chromophore within the purple nonfluorescent GFP-like chromoprotein asFP595 is synthesized. It is found that in water, the chromophere is found to exist in two forms, neutral and atomic, with a pK(sub a) of 7.1.

Published
2005

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