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Your search keyword '"Matsushita, Keizo"' showing total 2 results
Start Over Author "Matsushita, Keizo" Journal biochemistry
2 results on '"Matsushita, Keizo"'

1. Finger-positional change in three zinc finger protein Sp1: influence of terminal finger in DNA recognition

Author

Uno, Yumiko, Matsushita, Keizo, Nagaoka, Makoto, and Sugiura, Yukio

Subjects
Proteins -- Conformation, DNA-ligand interactions -- Analysis, Ligand binding (Biochemistry) -- Analysis, Conformational analysis -- Research, Biological sciences, Chemistry
Abstract

Research reveals that the wild-type and mutant zinc finger peptides bind to their respective binding sequences. However, the N-terminal finger of the mutant peptides exhibit varying degrees of base recognition due to the C-terminal finger induced conformational changes in the DNA.

Published
2001

2. Unique DNA binding mode of the N-terminal zinc finger of transcription factor Sp1

Author

Yokono, Masanori, Saegusa, Nana, Matsushita, Keizo, and Sugiura, Yukio

Subjects
Zinc -- Research, DNA -- Research, Genetic transcription -- Research, Nucleotides -- Research, Biological sciences, Chemistry
Abstract

Research was conducted to study the DNA recognition mode of human transcription factor Sp1 by examining the influence of zinc finger 1 to the high-affinity binding to the GC box DNA. Oligonucleotides were annealed and inserted between a BamHI site and an EcoRI site while mutated GC box sequences were utilized to analyze the DNA sequence selectivity of finger 1. Results showed that the interaction mechanism between finger 1 and DNA differs from the Zif268 model.

Published
1998

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