1. A ribonucleic acid-iodo-proteinaceous complex from the thyroid
- Author
-
Fredrick J. Kull and Morris Soodak
- Subjects
Hot Temperature ,Swine ,Thyroid Gland ,Sodium Chloride ,chemistry.chemical_compound ,RNA, Transfer ,Chemical Precipitation ,Amino Acids ,chemistry.chemical_classification ,Thyroid ,Hydrogen-Ion Concentration ,Amino acid ,medicine.anatomical_structure ,Biochemistry ,Spectrophotometry ,Transfer RNA ,Chromatography, Gel ,Diiodotyrosine ,Iodine ,Protein Binding ,Electrophoresis ,Paper ,Ultraviolet Rays ,chemistry.chemical_element ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Bile Acids and Salts ,Ribonucleases ,Phenols ,Microsomes ,medicine ,Centrifugation, Density Gradient ,Animals ,Nitrobenzenes ,Sheep ,Ethanol ,RNA ,Fluorine ,Alkaline Phosphatase ,Phosphoric Monoester Hydrolases ,Rats ,Thyroxine ,Enzyme ,chemistry ,Pronase ,Cattle ,Ultracentrifugation ,Hormone - Abstract
1. RNA-iodo-proteinaceous complexes occur in the tRNA fractions isolated from bovine, ovine, and porcine thyroids. The calf thyroid complex has been partially characterized and contains diiodotyrosine and probably the hormone thyroxine in addition to several other amino acids. 2. Different preparative procedures and techniques capable of disrupting a variety of non-covalent bonds and aminoacyl and peptidyl ester bonds had no obvious effect on the calf thyroid RNA-iodo-proteinaceous complex which remained associated with tRNA. 3. Nucleolytic enzymes can degrade the complex to lower molecular weight products that contain iodine, proteinaceous, and RNA components. However, most of the iodine and proteinaceous material is associated with a relatively high molecular weight, ribonuclease-resistant, RNA-containing core. 4. It is concluded that the tRNA fractions of bovine and probably other mammalian thyroid glands contain a low molecular weight RNA linked to proteinaceous material that contains iodinated amino acids.
- Published
- 1971