1. Combined enzymatic and chemical approaches to the synthesis of unique polyribonucleotides
- Author
-
John T. Uchic, Marie E. Uchic, and Arthur D. Broom
- Subjects
chemistry.chemical_classification ,Polyribonucleotide Nucleotidyltransferase ,Isostere ,Stereochemistry ,Phosphoric Diester Hydrolases ,Osmolar Concentration ,Polyribonucleotides ,Temperature ,chemistry.chemical_element ,Polymer ,Chromophore ,Alkaline Phosphatase ,Nucleic Acid Denaturation ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Sulfur ,Molecular Weight ,chemistry ,Polymerization ,medicine ,Nucleic Acid Conformation ,Spectrophotometry, Ultraviolet ,Polynucleotide phosphorylase ,Inosine ,medicine.drug - Abstract
The enzymatic polymerization by polynucleotide phosphorylase of 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-diphosphate to poly(6-chloropurinylic acid) and its conversion to poly(6-thioninosinic acid) is described. The sulfur isostere of poly(I) was found not to form a complex with poly(C), but to form a self-association complex with a Tm around 295 degrees K. The sedimentation velocities, pKa and Tm values of the polymer have been examined under various conditions. A two (or more) stranded helical array is suggested as the most probable structure. Thermal loss of the thione chromophore was noted for poly- (S6I), S6IMP and S6I; the degradation product from S6I was shown to be inosine.
- Published
- 1976