1. Purification, crystallisation and X-ray diffraction study of fully functional laccases from two ligninolytic fungi
- Author
-
Jean-Claude Sigoillot, Matteo Antorini, Kaspar H. Winterhalter, Klaus Piontek, Thomas Choinowski, Marcel Asther, Isabelle Herpoël-Gimbert, Unité mixte de recherche de biotechnologie des champignons filamenteux, Université de Provence - Aix-Marseille 1-Institut National de la Recherche Agronomique (INRA)-Université de la Méditerranée - Aix-Marseille 2, and ProdInra, Migration
- Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering ,[SDV]Life Sciences [q-bio] ,Biophysics ,chemistry.chemical_element ,Biochemistry ,Lignin ,law.invention ,03 medical and health sciences ,X-Ray Diffraction ,Structural Biology ,law ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Organic chemistry ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Crystallization ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,Trametes versicolor ,Laccase ,0303 health sciences ,biology ,030306 microbiology ,Basidiomycota ,Pycnoporus cinnabarinus ,[SDV.IDA] Life Sciences [q-bio]/Food engineering ,biology.organism_classification ,Chromatography, Ion Exchange ,Copper ,[SDV] Life Sciences [q-bio] ,Isoenzymes ,Crystallography ,chemistry ,X-ray crystallography ,Orthorhombic crystal system ,Isoelectric Focusing ,Oxidoreductases ,Monoclinic crystal system - Abstract
Laccase isozymes from the white-rot basidiomycete fungi Trametes versicolor and Pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. T. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group P2 1 2 1 2 1 , which diffract to 1.9 and 2.95 A resolution, respectively. The crystals of P. cinnabarinus laccase belong to the monoclinic space group C2 and diffract to at least 2.2 A resolution. All the laccase crystals are suitable for X-ray structure determination and contain a full complement of copper ions.
- Published
- 2002