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1. Nerve growth factor from Indian Russell's viper venom (RVV-NGFa) shows high affinity binding to TrkA receptor expressed in breast cancer cells: Application of fluorescence labeled RVV-NGFa in the clinical diagnosis of breast cancer

Author

Siddhartha Sankar Ghosh, Yuri N. Utkin, Rupak Mukhopadhyay, Ashis K. Mukherjee, Anil Parsram Bidkar, Taufikul Islam, and Munmi Majumder

Subjects
0301 basic medicine, Breast Neoplasms, Tropomyosin receptor kinase B, Viper Venoms, Tropomyosin receptor kinase A, Immunofluorescence, Biochemistry, Tropomyosin receptor kinase C, PC12 Cells, Gene Expression Regulation, Enzymologic, 03 medical and health sciences, Nerve Growth Factor, medicine, Animals, Humans, Receptor, trkA, Cytotoxicity, 030102 biochemistry & molecular biology, medicine.diagnostic_test, biology, Staining and Labeling, Chemistry, Optical Imaging, General Medicine, Molecular biology, Neoplasm Proteins, Rats, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Nerve growth factor, HEK293 Cells, nervous system, Snake venom, biology.protein, MCF-7 Cells, Nanoparticles, Female, Antibody, Fluorescein-5-isothiocyanate
Abstract

Nerve growth factor (NGF) is a minor and neglected component of snake venom. Present study describes the purification and characterization of a NGF isoform (RVV-NGFa) from Indian Russell’s viper venom (RVV). RVV-NGFa showed a protonated molecular ion [MH+] at m/z 17388.725 Da. The RVV-NGFa induced neuritogenesis in PC-12 cells but did not show cytotoxicity in mammalian cells, hemolytic activity, platelet modulation, and interference in blood coagulation system which are the characteristic pharmacological properties of RVV. By ELISA and immunofluorescence microplate reader assay, the RVV-NGFa showed appreciable binding to TrkA receptor expressed in breast cancer MDA-MB-231 and MCF-7 cells; nevertheless, pre-incubation of cells with anti-TrkA (and not TrkB or TrkC) or anti-p75NTR antibody significantly decreased (p

Published
2020

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