1. Expression of dengue ApoptoM sequence results in disruption of mitochondrial potential and caspase activation
- Author
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Santos A. Susin, Adeline Catteau, Victor J. Yuste, Philippe Desprès, Gaël Roué, Interactions Moléculaires Flavivirus-Hôtes, Institut Pasteur [Paris], Apoptose et Système Immunitaire (ASI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Apoptosis ,MESH: Amino Acid Sequence ,Mitochondrion ,MESH: Caspase 9 ,MESH: Dengue Virus ,Biochemistry ,Amino Acid Chloromethyl Ketones ,Membrane Potentials ,MESH: Recombinant Proteins ,MESH: Caspase 3 ,Caspase ,Caspase-9 ,0303 health sciences ,biology ,Caspase 3 ,030302 biochemistry & molecular biology ,General Medicine ,Caspase Inhibitors ,Caspase 9 ,Recombinant Proteins ,Mitochondria ,3. Good health ,Caspases ,MESH: Oligopeptides ,MESH: Luminescent Proteins ,Poly(ADP-ribose) Polymerases ,Oligopeptides ,Intracellular ,MESH: Enzyme Activation ,Programmed cell death ,MESH: Mitochondria ,Poly ADP ribose polymerase ,Green Fluorescent Proteins ,Molecular Sequence Data ,Cysteine Proteinase Inhibitors ,MESH: Cysteine Proteinase Inhibitors ,Viral Proteins ,03 medical and health sciences ,MESH: Green Fluorescent Proteins ,Humans ,MESH: Membrane Potentials ,Amino Acid Sequence ,030304 developmental biology ,MESH: Humans ,MESH: Molecular Sequence Data ,MESH: Caspases ,MESH: Apoptosis ,MESH: Poly(ADP-ribose) Polymerases ,MESH: Amino Acid Chloromethyl Ketones ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Dengue Virus ,Staurosporine ,MESH: Viral Proteins ,Molecular biology ,Enzyme Activation ,MESH: Hela Cells ,Luminescent Proteins ,MESH: Staurosporine ,biology.protein ,HeLa Cells - Abstract
Apoptotic cell death has been involved as a cytopathologic mechanism in response to dengue (DEN) virus infection. Little information exists about how DEN virus replication triggers apoptosis in infected cells. We reported that a nine-residue sequence of the DEN M protein referred to as ApoptoM has proapoptotic properties in transformed and tumor cells of various origins. The aim of the present study was to investigate whether ApoptoM-induced apoptosis is associated to mitochondrial dysfunction and requires caspase activation. Intracellular expression of ApoptoM provokes the disruption of the mitochondrial transmembrane potential without subsequent generation of reactive oxygen species. We showed that ApoptoM-induced apoptosis involves the activation of a caspase-like protease pathway. Caspase-3 like activity was detected in ApoptoM-expressing cells. However, there was no role for caspase-9 in ApoptoM-mediated cell death. Our data suggest that a particular mitochondrion-dependent apoptotic pathway may be involved in induction of apoptosis by ApoptoM.
- Published
- 2003
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