1. Pilot-scale production of mosquitocidal toxins by Bacillus thuringiensis and Lysinibacillus sphaericus under solid-state fermentation.
- Author
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El-Bendary, Magda A., Moharam, Maysa E., Mohamed, Sahar S., and Hamed, Shimaa R.
- Subjects
BACILLUS (Bacteria) ,SOLID-state fermentation ,SUGAR beets ,TOXICITY testing ,INDUSTRIAL wastes - Abstract
Mosquitocidal toxins ofBacillus thuringiensis israelensis(Bti) andLysinibacillus sphaericus14N1 (Ls14N1) were produced under solid-state fermentation using agro-industrial wastes. Sugar beet pulp–sesame meal (1:1) and wheat germ meal–linen meal (1:1) at 9% were the efficient substrate mixtures for the growth and toxin production of Bti and Ls14N1, respectively. Bti was more active after the addition of beef extract (0.2%) or yeast extract (0.5%) to the medium. On the other hand, the addition of yeast extract (0.2%) or NYSM salts (2%) significantly enhanced the toxicity produced by Ls14N1. The optimum conditions for the maximum toxicity of Bti were at pH 7–8, 20–30% moisture, 4–10% inoculum and 7 days incubation. For Ls14N1, the best conditions were pH 6.5–7.5, 20–30% moisture, 4–10% inoculum and 5 days incubation. It was found that the best thickness of carrier-substrates in the plate (15 cm in diameter) for the maximum mosquitocidal activity was about 0.5 cm for Bti and 0.5–1 cm for Ls14N1. Pilot-scale production in aluminium trays applying the above conditions showed a decrement of toxicity of fermented cultures and some plates were contaminated. These problems were dissolved by reducing the moisture content to 15%, increasing inoculum to 10% and manual agitation of trays every-day. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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