1. Aerobic biodegradation of propylene glycol by soil bacteria
- Author
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Giuseppe Toscano, M. Letizia Colarieti, Guido Greco, Rosalia Scelza, Riccardo Scotti, Maria A. Rao, Sonia Ciccazzo, Vincenza Andreoni, Lucia Cavalca, Toscano, Giuseppe, L., Cavalca, M., Letizia Colarieti, Scelza, Rosalia, Scotti, Riccardo, Rao, MARIA ANTONIETTA, V., Andreoni, S., Ciccazzo, and Greco, Guido
- Subjects
Environmental Engineering ,Bioengineering ,010501 environmental sciences ,01 natural sciences ,Microbiology ,Polymerase Chain Reaction ,Biostimulation ,Bacteria (microorganisms), Bacteroidetes, Pseudomonas ,03 medical and health sciences ,Soil ,Bioremediation ,Nutrient ,Norway, Oslo [Norway] ,Pseudomonas ,RNA, Ribosomal, 16S ,Aircraft de-icing fluid, Biodegradation kinetics, Propylene glycols, Pseudomonas, Soil bioremediation ,Environmental Chemistry ,Cluster Analysis ,Aerobic bacteria ,Soil Microbiology ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,Bacteria ,Chemistry ,Denaturing Gradient Gel Electrophoresis ,Biodegradation, Bioremediation, Carbon, Groundwater, Microbiology, Polyols, Soil ,Soil classification ,Sequence Analysis, DNA ,Biodegradation ,Pollution ,Soil contamination ,Propylene Glycol ,Aerobiosis ,Kinetics ,Biodegradation, Environmental ,Environmental chemistry ,Soil horizon ,aircraft, bacterium, biodegradation, bioremediation, groundwater pollution, growth rate, nutrient limitation, oxic conditions, pollutant source, soil microorganism, soil pollution, soil profile, substrate ,Energy source - Abstract
Propylene glycol (PG) is a main component of aircraft deicing fluids and its extensive use in Northern airports is a source of soil and groundwater contamination. Bacterial consortia able to grow on PG as sole carbon and energy source were selected from soil samples taken along the runways of Oslo Airport Gardermoen site (Norway). DGGE analysis of enrichment cultures showed that PG-degrading populations were mainly composed by Pseudomonas species, although Bacteroidetes were found, as well. Nineteen bacterial strains, able to grow on PG as sole carbon and energy source, were isolated and identified as different Pseudomonas species. Maximum specific growth rate of mixed cultures in the absence of nutrient limitation was 0.014 h(-1) at 4 °C. Substrate C:N:P molar ratios calculated on the basis of measured growth yields are in good agreement with the suggested values for biostimulation reported in literature. Therefore, the addition of nutrients is suggested as a suitable technique to sustain PG aerobic degradation at the maximum rate by autochthonous microorganisms of unsaturated soil profile.
- Published
- 2012