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Soesanto L, Susanti RA, Mugiastuti E, Manan A, Sastyawan MWR, Maryanto J. 2022. Remediation of chlorpyrifos-contaminated soils by crude secondary metabolites of Trichoderma harzianum T213 and its effect on maize growth. Biodiversitas 23: 2464-2470. The study was conducted to determine the role of Trichoderma harzianum T213 crude secondary metabolites on the growth of maize plants and the reduction of chlorpyrifos on contaminated soils. A randomized block design was used in this study with four treatments. The treatments applied on chlorpyrifos contaminated soil were: without the application of secondary metabolites (0%) and the application of T. harzianum T213 crude secondary metabolites at the concentration of 50, 100, and 150%. Variables observed were residual levels of insecticide in maize, plant height difference, number of leaves, plant fresh weight, and root length. The results showed that the application of T. harzianum T213 crude secondary metabolites effectively increased plant growth, including plant height, the number of leaves, plant fresh weight, and root length compared to control on chlorpyrifos-contaminated soil. The highest percentage (99.54%) of the reduction of chlorpyrifos residue was the application of 100% T. harzianum T213 crude secondary metabolites. It was followed by applying 50 and 150% T. harzianum T213 crude secondary metabolites by 99.39 and 99.14%, respectively.

Cano-Reinoso DM, Soesanto L, Kharisun, Wibowo C. 2021. Review: Fruit collapse and heart rot disease: Pathogen characterization, ultrastructure infections of plant and cell mechanism resistance. Biodiversitas 22: 2477-2488. Fruit collapse and bacterial heart rot are diseases in pineapple caused by Erwinia chrysanthemi (later classified as Dickeya zeae) which are increasingly prevalent in the last decade, causing devastating production loss in pineapple cultivation. Yet, comprehensive knowledge to tackle such diseases is limited, understandably due to the relatively new emerge of the diseases. Here, we review the causes of bacterial heart rot and fruit collapse, stages of infection, typical symptoms and the occurrence of resistance mechanisms in plants. In pineapple, the fruit collapse is noticeable by the release of juice and gas bubbles, also the shell of the fruit that turns into olive-green. Meanwhile, bacterial heart rot is characterized by water-soaked zones on the leaves, the formation of brown streaks on the lamina and in the mesophyll, and light-brown exudate in the blisters. The most common means of penetration into the host plant used by this type of pathogen is through plant natural openings, injuries and wounds, and entire surfaces. Concurrently, plants and fruits develop disease-resistant mechanisms to inhibit infection growth under this pathogenic attack. These mechanisms can be divided into hypersensitive reactions, locally acquired resistance, and systematic acquired resistance. In addition, pathological infections produce an interaction of the cell wall with pectolytic enzymes. Understanding the membrane breakdown process carried out by these enzymes has become critical to a pineapple protection plan. This review suggests that future research to tackle fruit collapse and bacterial heart rot can be focused on disease-resistant mechanisms, and their effects on the cell wall status with an enzymatic characterization.

Muljowati JS, Soesanto L, Nugroho LH. 2021. Short Communication: Histopathology of red chilli fruit (Capsicum annuum) infected with Colletotrichum acutatum of Java, Indonesia isolates. Biodiversitas 22: 874-880. Colletotrichum acutatum isolates from Malang, Temanggung, Kulonprogo, Brebes, Garut, and Pandeglang in Indonesia varied in their ability to produce pigments. In the present study, the histopathological status of red chilli was investigated during the early phase of infection by C. acutatum Java isolate. The results included a description of the histopathological features of red chillies (Capsicum annuum) in the early phase of infection by C. acutatum isolates and the relationship between the origin of the isolates and the time of onset of infection. The red chilli fruits were inoculated with fungal conidia suspension and then the histopathology of chilli fruits was observed at 0 hours (uninfected control), 8 hours, 16 hours, 24 hours, and 32 hours after inoculation. The results showed that C. acutatum isolates from Kulonprogo caused host damage within 8 hours, while the other isolates (from Malang, Brebes, Garut and Pandeglang) began to produce similar symptoms at 16 hours or 24 hours. This study revealed for the first time that pathogenic activity begins at the onset of infection, and the resulting anatomical damage to red chillies begins at different times.

Soesanto L, Mugiastuti E, Manan A. 2021. The use of alternative liquid media for propagation of pathogenic fungi and their effect on weeds. Biodiversitas 22: 719-725. Weeds cause high yield losses and are controlled by chemical, physical and mechanical control. Weed pathogenic fungi could be used as a biological control agent. However, the propagation media of them are still a higher cost, therefore, the new alternative and inexpensive media for weed pathogenic fungi propagation are required. The propagation of weed pathogenic fungi in liquid media in-vitro used randomize completely design consisted of three pathogenic weed fungi, i.e., Chaetomium sp., Fusarium sp., and Curvularia lunata and three liquid media, i.e., PDB, rice washing water, and tofu liquid waste. The in-planta tests used split-plot design used weed pathogenic fungi multiplied in the best formula from in-vitro results (rice washing water) and control as the main plot and four weeds, i.e., Cyperus kyllingia, Cynodon dactylon, Portulaca oleracea, and Ageratum conyzoides as subplot. The variables observed were conidia density, incubation period, disease intensity, number of leaves, and weed height. The results showed that rice washing water and tofu liquid waste were suitable media for the propagation of the weed pathogenic fungi. Chaetomium sp. was virulent fungi against broad leaves weeds, especially A. conyzoides. This fungus can be promoted to manufacture organic herbicides against the weed.

Soesanto L, Fatihah B, Manan A, Mugiastuti E, Prihaningsih N. 2020. Organic control of Bemisia tabaci Genn. on Capsicum annuum with entomopathogenic fungi raw secondary metabolites. Biodiversitas 21: xxxx. Control of whitefly with entomopathogenic fungi raw secondary metabolites and their correlation was investigated under control condition. The in vitro test used randomize completely design consisted of five treatments (raw secondary metabolites of Beauveria bassiana B16 and B10, Metarhizium anisopliae M16, Lecanicillium lecanii L16, and no treatments) and repeated five times. The in planta one used Latin square design consisted of five treatments and repeated five times. Variables observed were the mortality of whitefly imago, chitinolytic index, incubation period, viral disease intensity, plant height and total leaves per plant. The results showed that all treatment of raw secondary metabolites of entomopathogenic fungi caused mortality in the B. tabaci imago by 77.8% (B. bassiana B16); 71.5% (B. bassiana B10); 66.7% (M. anisopliae M16); and 59.5% (L. lecanii L16). All raw secondary metabolites of entomopathogenic fungi reduce the intensity of viral diseases by 79.98% (B. bassiana B16); 74.00% (B. bassiana B10); 72.23% (L. lecanii L16); and 55.41% (M. anisopliae M16). The correlation between B. tabaci mortality due to raw secondary metabolites of entomopathogenic fungi with the viral disease intensity is very close and opposite. Every 1% increase in pest mortality causes decrease in viral intensity by 1.346%.

Maulidia V, Soesanto L, Syamsuddin, Khairan K, Hamaguchi T, Hasegawa K, Sriwati R. 2020. Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.). Biodiversitas 21: 5270-5275. Endophytic bacteria live and colonize in plant tissues without causing disease to their plant host. Among several processes, these bacteria can produce secondary metabolites that can help in the defense of plant host against pathogens. This study aimed to identify endophytic bacteria as biocontrol agents against Meloidogyne sp. in tomato plants. Six endophytic bacteria candidates from the genus Pseudomonas, Arthrobacter, Bacillus, and Serratia were isolated from Solanum Lycopersicum, Psidium guajava, Pinus merkusii, Dendrocalamus asper, Albizia chinensis, and Theobroma cacao L, respectively. The average mortality of Meloidogyne sp. by endophytic bacteria was 70,27% to 95,46%. From these, B. thuringiensis AK08 produced compounds of the secondary metabolites such as flavonoid, phenol, tannins, terpenoids, steroids, saponins, and alkaloids. The best result of the average incubation period, number of galls in the root, number of nematodes at the root, and the number of nematodes in the soil on tomato plant were shown by B. thuringiensis. The major compounds in GC-MS analysis of B. thuringiensis were cholest-5-en-3-ol (3.beta.)-carbonochloridate (25.35%). Bacillus thuringiensis not only has rules as bio-insecticide but also has nematicidal effect.

Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Short Communication: Isolation And Characterization Of The Endophytic Bacteria, And Their Potential As Maize Diseases Control. Biodiversitas 21: 1809-1815. Sheath blight and bacterial wilt are diseases that can reduce maize production. Biological control with the endophytic bacteria offers environmentally friendly control for these pathogens. The study aimed to isolate and characterize the endophytic bacteria morphologically and biochemically and to study their potential to control maize diseases, especially sheat blight and bacterial wilt causing pathogens. The study was conducted at the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University, from April to August 2019. The study consisted of four stages: isolation and characterization of endophytic bacteria, the antagonism test of the endophytic bacterial to R solani, the antagonism test of the endophytic bacteria to Pantoea sp., and the mechanical test of the endophytic bacteria as biological control agents and plant growth-promoting bacteria. Based on the research, four endophytic bacteria isolates have been successfully isolated, and characterized successfully and found have the potential to be developed as biopesticides to control maize disease, especially R. solani and Pantoea sp. Bacillus sp, endophytic from the root (BK.A1; BK.A3; PP.A5) and Bacillus sp. endophytic from the stem (PPD.B2) can suppress the growth of R.solani by more than 50%, have a strong antagonistic index against Pantoea sp (> 4), and can produce protease and lipase enzyme, and phosphate solubilization.