1. Exogenous gene integration mediated by genome editing technologies in zebrafish
- Author
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Hitoshi Morita, Atsuo Kawahara, Kiyohito Taimatsu, and Kanoko Yanagi
- Subjects
0301 basic medicine ,ved/biology.organism_classification_rank.species ,Bioengineering ,Locus (genetics) ,Biology ,Applied Microbiology and Biotechnology ,Genome ,Animals, Genetically Modified ,03 medical and health sciences ,Genome editing ,Gene knockin ,CRISPR ,Animals ,Clustered Regularly Interspaced Short Palindromic Repeats ,Model organism ,Gene ,Zebrafish ,Genetics ,Gene Editing ,Transcription activator-like effector nuclease ,Review CRISPR Special Focus ,ved/biology ,fungi ,General Medicine ,Zebrafish Proteins ,030104 developmental biology ,CRISPR-Cas Systems ,Biotechnology - Abstract
Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in the process of DSB repair. Recently, the technology-induced DSBs followed by DSB repairs are applied to integrate exogenous genes into the targeted genomic locus in various model organisms. In addition to a conventional knock-in technology mediated by homology-directed repair (HDR), novel knock-in technologies using refined donor vectors have also been developed with the genome editing technologies based on other DSB repair mechanisms, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). Therefore, the improved knock-in technologies would contribute to freely modify the genome of model organisms.
- Published
- 2017