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The use of new autonomous and Lagrangian platforms (e.g. gliders, drifters, etc.) has revolutionized sampling of the ocean. The incorporation of in vivo chlorophyll-a fluorometers into these platforms for characterizing chlorophyll-a concentrations and phytoplankton biomass has reinforced the need for a thorough 5 understanding of the variability and biases associated with basic fluorescence measurements. Seaglider, a long-range autonomous glider, has been deployed routinely in Northeast Pacific waters off the Washington coast, USA. Measurements of chlorophyll-a fluorescence (proxy for chlorophyll-a concentration) and optical backscattering (proxy for particle concentration) were collected on the continental shelf and along a V-shaped transect that extended 200 km from the continental shelf into deep oceanic waters. Daytime fluorescence quenching (i.e. the reduction in the fluorescence quantum yield often observed during daylight hours) could be detected throughout the dataset, with near-surface daytime fluorescence quenched by as much as 80% during summer. Quenching was 15 observed throughout the region, at all times of year, and to depths greater than 50 m. The degree of quenching was positively correlated with incoming solar radiation and the observed pattern was remarkably similar to what has been observed in other areas, suggesting some degree of universality for the underlying relationship. [ABSTRACT FROM AUTHOR]