1. Idesolide, an Isolate of Idesia polycarpa, Inhibits Apoptosis through Induction of Intracellular Heat Shock Protein 70 in C2C12 Muscle Cells
- Author
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Yeo Jin Kang, Hyoung Woo Lee, Yong Jin Lee, Tack Joong Kim, Jung min Yoo, Min Ho Jung, Inho Choi, Seung Hyun Kim, and Sang Hyun Sung
- Subjects
Programmed cell death ,Salicaceae ,Cell Survival ,Pharmaceutical Science ,Apoptosis ,Muscle disorder ,Biology ,Antioxidants ,Cell Line ,Mice ,Atrophy ,Heat shock protein ,medicine ,Animals ,Myocyte ,HSP70 Heat-Shock Proteins ,Spiro Compounds ,Muscular dystrophy ,Pharmacology ,Muscle Cells ,Plant Extracts ,Hydrogen Peroxide ,General Medicine ,medicine.disease ,Muscle atrophy ,Cell biology ,Muscular Atrophy ,Oxidative Stress ,Biochemistry ,medicine.symptom ,Intracellular ,Phytotherapy - Abstract
Muscle disorders, such as muscular dystrophy, are associated with an increase in oxidative stress. Proposed treatments for muscular dystrophy, some in clinical trials, include gene therapy and muscle cell transplantation. In this study, we investigated the effects of idesolide, isolated from the fruits of Idesia polycarpa, on changes that occur in muscle disuse atrophy. We noted protective effects on oxidative stress response and HSP70 regulation. Pre-treatment with idesolide for 24 h maintained cell viability and decreased apoptosis in H(2)O(2)-treated C(2)C(12) muscle cells. The idesolide pretreatment also increased intracellular HSP70 protein. Our results suggest that idesolide inhibits cell death through induction of HSP70 in C(2)C(12) muscle cells. This work is the first to report that idesolide can regulate the decrease in HSP70 that occurs during skeletal muscle atrophy.
- Published
- 2010
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