1. Mouse Aortic Ring Assay: A New Approach of the Molecular Genetics of Angiogenesis
- Author
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Peter Carmeliet, Christine Grignet-Debrus, Jean-Michel Foidart, Agnès Noël, Véronique Masson, Guy Roland, Silvia Blacher, Yawen Chang, Laetitia Devy, Khalid Bajou, Timothy Fong, and Sarah Bernt
- Subjects
medicine.medical_specialty ,endothelium ,Angiogenesis ,Transgene ,Proteolysis ,Biology ,Matrix metalloproteinase ,General Biochemistry, Genetics and Molecular Biology ,Angiogenesis factor ,law.invention ,Extracellular matrix ,law ,Molecular genetics ,medicine ,lcsh:QH301-705.5 ,mouse ,chemistry.chemical_classification ,lcsh:R5-920 ,medicine.diagnostic_test ,Biochemistry, Genetics and Molecular Biology(all) ,Cell biology ,Enzyme ,chemistry ,Biochemistry ,lcsh:Biology (General) ,Recombinant DNA ,lcsh:Medicine (General) ,Research Article - Abstract
Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen "pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer.
- Published
- 2002