1. Comparative analysis of the protective effects of caffeic acid phenethyl ester (CAPE) on pulmonary contusion lung oxidative stress and serum copper and zinc levels in experimental rat model
- Author
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Okan Solak, Namik Delibas, Zeynep Ginis, Cagatay Tezel, Halis Köylü, Yetkin Agackiran, Rana Sirmali, Dilek Atik, and Mehmet Sırmalı
- Subjects
Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Contusions ,Clinical Biochemistry ,medicine.disease_cause ,Protective Agents ,Biochemistry ,Gastroenterology ,Inorganic Chemistry ,Superoxide dismutase ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Random Allocation ,Caffeic Acids ,Internal medicine ,Cape ,Malondialdehyde ,medicine ,Animals ,Humans ,Caffeic acid phenethyl ester ,Lung ,Analysis of Variance ,biology ,business.industry ,Superoxide Dismutase ,Biochemistry (medical) ,General Medicine ,Phenylethyl Alcohol ,medicine.disease ,Catalase ,Surgery ,Rats ,Pulmonary contusion ,Disease Models, Animal ,Oxidative Stress ,Zinc ,medicine.anatomical_structure ,chemistry ,biology.protein ,Blood Gas Analysis ,business ,Oxidative stress ,Copper - Abstract
The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) in the lungs by biochemical and histopathological analyses in an experimental isolated lung contusion model. Eighty-one male Sprague-Dawley rats were used. The animals were divided randomly into four groups: group 1 (n = 9) was defined as without contusion and without CAPE injection. Group 2 (n = 9) was defined as CAPE 10 mu mol/kg injection without lung contusion. Group 3 (n = 36) was defined as contusion without CAPE-administrated group which consisted of four subgroups that were created according to analysis between days 0, 1, 2, and 3. Group 4 (n = 27) was defined as CAPE 10 mu mol/kg administrated after contusion group divided into three subgroups according to analysis on days 1, 2, and 3. CAPE 10 mu mol/kg was injected intraperitoneally 30 min after trauma and on days 1 and 2. Blood samples were obtained to measure catalase (CAT) and superoxide dismutase (SOD) activities and level of malondialdehyde (MDA) and for blood gas analysis. Trace elements such as zinc and copper were measured in serum. The lung tissue was also removed for histopathological examination. Isolated lung contusion increased serum and tissue SOD and CAT activities and MDA levels (p < 0.05). Both serum and tissue SOD, MDA, and CAT levels on day 3 were lower in group 4 compared to group 3 (p < 0.05). Further, the levels of SOD, MDA, and CAT in group 4 were similar compared to group 1 (p > 0.05). CAPE also had a significant beneficial effect on blood gases (p < 0.05). Both serum zinc and copper levels were (p < 0.05) influenced by the administration of CAPE. Histopathological examination revealed lower scores in group 4 compared to group 3 (p < 0.05) and no significant differences compared to group 1 (p > 0.05). CAPE appears to be effective in protecting against severe oxidative stress and tissue damage caused by pulmonary contusion in an experimental setting. Therefore, we conclude that administration of CAPE may be used for a variety of conditions associated with pulmonary contusion. Clinical use of CAPE may have the advantage of prevention of pulmonary contusion.
- Published
- 2012