1. Peptidylglycine α-amidating monooxygenase activity and TRH and CRF biosynthesis
- Author
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Françpise Boudouresque, L'Houcine Ouafik, Pierre Giraud, J. Y. Maltese, P. Salers, Charles Oliver, François Pelen, M Renard, INSERM U 297 - Laboratoire de Neuroendocrinologie Expérimentale, Institut National de la Santé et de la Recherche Médicale (INSERM), and Ouafik, L'Houcine
- Subjects
Corticotropin-Releasing Hormone ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Thyrotropin-releasing hormone ,MESH: Amino Acid Sequence ,MESH: Multienzyme Complexes ,MESH: Chelating Agents ,Biochemistry ,Mixed Function Oxygenases ,chemistry.chemical_compound ,Corticotropin-releasing hormone ,Insulin ,MESH: Animals ,Thyrotropin-Releasing Hormone ,Cells, Cultured ,Chelating Agents ,Biological activity ,General Medicine ,MESH: Mixed Function Oxygenases ,MESH: Corticotropin-Releasing Hormone ,MESH: Copper ,Hypothalamus ,Ditiocarb ,Peptidylglycine alpha-amidating monooxygenase ,MESH: Ditiocarb ,hormones, hormone substitutes, and hormone antagonists ,MESH: Cells, Cultured ,endocrine system ,MESH: Rats ,Molecular Sequence Data ,Neuropeptide ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,MESH: Insulin ,Inorganic Chemistry ,Islets of Langerhans ,Fetus ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Biosynthesis ,MESH: Thyrotropin-Releasing Hormone ,Multienzyme Complexes ,Animals ,Amino Acid Sequence ,MESH: Molecular Sequence Data ,MESH: Islets of Langerhans ,Biochemistry (medical) ,MESH: Fetus ,Monooxygenase ,MESH: Hypothalamus ,Rats ,chemistry ,Copper - Abstract
International audience; Carboxy-terminal amidation of biologically active peptides, an important characteristic of more than half of these substances, occurs during the maturation process of peptide precursors. It is catalyzed by peptidylglycine alpha-amidating monooxygenase (PAM), an enzyme that is copper-dependent. We show here that alterations of copper stores in cultured cells from different origins (pancreas and hypothalamus) affect the immunoreactivity of thyrotropin-releasing hormone (TRH) and corticotropin-releasing factor (CRF) (two alpha-amidated peptides). This suggests that copper can affect neuropeptide biosynthesis and may play a role in the endocrine or central nervous system function.
- Published
- 1992