1. Development of a new, simple, rapid ultra-high-performance liquid chromatography (UHPLC) method for the quantification of 2-phenoxyethanol in vaccines for human use
- Author
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Andrea Battistone, Francesca Luciani, Christina von Hunolstein, Francesco Marino, and Valeria Esposito
- Subjects
0301 basic medicine ,Acetonitriles ,Materials science ,Calibration curve ,Bioengineering ,Applied Microbiology and Biotechnology ,2 phenoxyethanol ,Phenoxyethanol ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Phase (matter) ,Humans ,030212 general & internal medicine ,Acetonitrile ,Chromatography, High Pressure Liquid ,Pharmacology ,Vaccines ,Chromatography ,General Immunology and Microbiology ,Preservatives, Pharmaceutical ,General Medicine ,Quantitative determination ,030104 developmental biology ,chemistry ,Ethylene Glycols ,Analytical procedures ,Ultra high performance ,Biotechnology - Abstract
A new, simple and rapid method for the quantitative determination of the antimicrobial preservative 2-phenoxyethanol, based on reverse phase ultra-high-performance liquid chromatography has been developed. The validation was performed according the ICH Q2 guideline “Validation of Analytical Procedures”. The desired chromatographic separation was achieved on a Waters Symmetry C18 (150 × 4.6 mm, 5 μm) column using an isocratic elution, with detection at 270 nm wavelength. The mobile phase consisted of acetonitrile/water (55:45, v/v), pumped at a flow rate of 1 mL/min. The calibration curve and the analytical procedure are linear (r2 = 0.999) from the concentration of 0.07 mg/mL to 1.1 mg/mL. The percent relative standard deviation for intra- and inter-day precision was
- Published
- 2021
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