1. Blocking adherence of uropathogenic Escherichia coli isolate to HEP-2 cells and bladder of mice in the presence of antibody against p-fimbriae.
- Author
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Bidhendi SM, Sattari M, Pourbakhsh SA, Mobarez A, Vandyousefi J, Khaki P, Heidari MH, and Kazemnejad A
- Subjects
- Animals, Antibodies pharmacology, Cells, Cultured, Epithelial Cells immunology, Epithelial Cells microbiology, Escherichia coli Infections microbiology, Escherichia coli Infections urine, Female, Fimbriae Proteins isolation & purification, Fimbriae Proteins urine, Fimbriae, Bacterial immunology, Humans, Larynx immunology, Mice, Mice, Inbred Strains, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Rabbits, Urinary Bladder immunology, Urinary Tract Infections microbiology, Urinary Tract Infections urine, Bacterial Adhesion immunology, Escherichia coli immunology, Escherichia coli isolation & purification, Fimbriae Proteins immunology, Larynx microbiology, Urinary Bladder microbiology
- Abstract
Presence of fimbriae on Escherichia coli isolated from the urine of patients with urinary tract infection was related to the ability of the bacteria to attach to human uroepithelial cells. One of the 50 isolates that expresses high MRHA p-fimbriae, selected and antibody against p-fimbriae from it, showed blocking of attachment of bacteria to HEP-2 cell in 1:1024 titer. Also, 1:512 titer of this antiserum to blocking of attachment in bladder tissue of mice is significant.
- Published
- 2007
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