1. Changes in intracellular pH following egg activation and during the early cell cycle of the amphibian Pleurodeles waltlii, coincide with changes in MPF activity
- Author
-
Nathalie Grandin, Jean-Paul Rolland, Michel Charbonneau, Laboratoire d'Innovation pour les Technologies des Energies Nouvelles et les nanomatériaux (LITEN), Institut National de L'Energie Solaire (INES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie et Génétique du Développement, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), and Grandin, Nathalie
- Subjects
MESH: Hydrogen-Ion Concentration ,Time Factors ,[SDV]Life Sciences [q-bio] ,Maturation-Promoting Factor ,Xenopus ,MESH: Cell Cycle ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,0302 clinical medicine ,Protein biosynthesis ,MESH: Animals ,MESH: Cycloheximide ,Cycloheximide ,0303 health sciences ,biology ,MESH: Kinetics ,Cell Cycle ,General Medicine ,Anatomy ,Cell cycle ,Hydrogen-Ion Concentration ,Cell biology ,Phosphorylation ,Female ,Amphibian ,Pleurodeles ,MESH: Adenine ,Intracellular pH ,Mitosis ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,MESH: Pleurodeles ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,MESH: Oocytes ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,biology.animal ,[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,Animals ,030304 developmental biology ,urogenital system ,Adenine ,MESH: Maturation-Promoting Factor ,MESH: Time Factors ,Oocyte activation ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,MESH: Mitosis ,biology.organism_classification ,Kinetics ,Oocytes ,MESH: Female ,030217 neurology & neurosurgery - Abstract
International audience; Previous work on Xenopus laevis suggests a temporal coincidence between inactivation of the M-phase promoting factor (MPF) and intracellular pH (pHi) increase during egg activation. In addition, we recently showed that during the early cell cycle of Xenopus eggs, MPF activity cycling and pHi oscillations were temporally and functionally related. In the present work, using eggs of another amphibian, Pleurodeles waltlii, which has a natural cell cycle considerably longer than that of Xenopus laevis, we show a temporal coincidence between MPF activity and pHi changes, both at the time of egg activation and at each of the following cell cycles. Egg activation-induced pHi changes in Pleurodeles did not involve classical plasma membrane ion exchangers, and were not due to the activation of a H+ conductance. On the other hand, the pHi oscillations intervening at each cell cycle were suppressed by inhibitors of protein synthesis or phosphorylation, as were their counterparts in Xenopus eggs. We propose that physiological pHi changes in Pleurodeles and Xenopus eggs might have a metabolic origin, in direct relation with the cascade of phosphorylations-dephosphorylations of proteins implicated in the control of the cell cycle.
- Published
- 1991