1. Photoluminescent Hybrids of Cellulose Nanocrystals and Carbon Quantum Dots as Cytocompatible Probes for in Vitro Bioimaging.
- Author
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Guo J, Liu D, Filpponen I, Johansson LS, Malho JM, Quraishi S, Liebner F, Santos HA, and Rojas OJ
- Subjects
- Animals, HeLa Cells, Humans, Mice, Microscopy, Fluorescence methods, RAW 264.7 Cells, Cellulose chemistry, Cellulose pharmacology, Fluorescent Dyes chemistry, Fluorescent Dyes pharmacology, Materials Testing, Nanoparticles chemistry, Quantum Dots chemistry
- Abstract
We present an approach to construct biocompatible and photoluminescent hybrid materials comprised of carbon quantum dots (CQDs) and TEMPO-oxidized cellulose nanocrystals (TO-CNCs). First, the amino-functionalized carbon quantum dots (NH
2 -CQDs) were synthesized using a simple microwave method, and the TO-CNCs were prepared by hydrochloric acid (HCl) hydrolysis followed by TEMPO-mediated oxidation. The conjugation of NH2 -CQDs and TO-CNCs was conducted via carbodiimide-assisted coupling chemistry. The synthesized TO-CNC@CQD hybrid nanomaterials were characterized using X-ray photoelectron spectroscopy, cryo-transmittance electron microscopy, confocal microscopy, and fluorescence spectroscopy. Finally, the interactions of TO-CNC@CQD hybrids with HeLa and RAW 264.7 macrophage cells were investigated in vitro. Cell viability tests suggest the surface conjugation with NH2 -CQDs not only improved the cytocompatibility of TO-CNCs, but also enhanced their cellular association and internalization on both HeLa and RAW 264.7 cells after 4 and 24 h incubation.- Published
- 2017
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