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Start Over Topic laccase Journal biomacromolecules
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1. Printing of polymer microcapsules for enzyme immobilization on paper substrate.

Author

Savolainen A, Zhang Y, Rochefort D, Holopainen U, Erho T, Virtanen J, and Smolander M

Subjects
Biotechnology methods, Capsules chemistry, Capsules metabolism, Drug Compounding methods, Enzymes, Immobilized chemistry, Fungal Proteins chemistry, Ink, Laccase chemistry, Lignin metabolism, Paper, Polyethyleneimine metabolism, Trametes chemistry, Enzymes, Immobilized metabolism, Fungal Proteins metabolism, Laccase metabolism, Polyethyleneimine chemistry, Printing methods, Trametes enzymology
Abstract

Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta (ThL) and Trametes versicolor (TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime.

Published
2011
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2. Printing of Polymer Microcapsules for Enzyme Immobilization on Paper Substrate.

Author

Anne Savolainen, Yufen Zhang, Dominic Rochefort, Ulla Holopainen, Tomi Erho, Jouko Virtanen, and Maria Smolander

Subjects
*POLYMERS, *MICROSTRUCTURE, *IMMOBILIZED enzymes, *PAPER, *SUBSTRATES (Materials science), *AZIRIDINES, *LACCASE, *TRAMETES versicolor
Abstract

Poly(ethyleneimine) (PEI) microcapsules containing laccase from Trametes hirsuta(ThL) and Trametes versicolor(TvL) were printed onto paper substrate by three different methods: screen printing, rod coating, and flexo printing. Microcapsules were fabricated via interfacial polycondensation of PEI with the cross-linker sebacoyl chloride, incorporated into an ink, and printed or coated on the paper substrate. The same ink components were used for three printing methods, and it was found that laccase microcapsules were compatible with the ink. Enzymatic activity of microencapsulated TvL was maintained constant in polymer-based ink for at least eight weeks. Thick layers with high enzymatic activity were obtained when laccase-containing microcapsules were screen printed on paper substrate. Flexo printed bioactive paper showed very low activity, since by using this printing method the paper surface was not fully covered by enzyme microcapsules. Finally, screen printing provided a bioactive paper with high water-resistance and the highest enzyme lifetime. [ABSTRACT FROM AUTHOR]

Published
2011
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3. Increased water resistance of CTMP fibers by oat (Avena sativa L.) husk lignin

Author

Mika Henrikki Sipponen, Isto T. Heiskanen, Ossi Pastinen, Reetta Strengell, Simo Laakso, and Juha M. I. Hyötyläinen

Subjects
Paper, Absorption of water, food.ingredient, Polymers and Plastics, Avena, Bioengineering, engineering.material, Husk, Lignin, Biomaterials, Contact angle, chemistry.chemical_compound, food, Adsorption, Materials Chemistry, Laccase, Chemistry, Pulp (paper), Water, Chemical engineering, Biochemistry, engineering, Hydrophobic and Hydrophilic Interactions
Abstract

The insertion of oat husk lignin onto chemithermomechanical pulp (CTMP) fibers was studied to increase fiber hydrophobicity. The pretreated pulp samples were subsequently used for preparation of handsheets for characterization. Treatment of CTMP with laccase in the presence of oat husk lignin resulted in a significant increase in hydrophobicity of the handsheet surface, as indicated by dynamic contact angle analysis. Water absorption time of 8 s was obtained with initial contact angle of 118°. Although the handsheet's brightness was reduced by 33%, tensile index was only subtly decreased. Neither laccase nor oat husk lignin alone gave much improved water absorption times. Therefore, handsheets made of laccase-treated pulp with and without oat husk lignin were further examined by XPS, which suggested that both laccase and oat husk lignin were inserted onto CTMP fibers. The oat husk lignin was distributed as heterogeneous aggregates on the handsheet surface whereas laccase was uniformly distributed. Evidence was obtained that the adsorbed laccase layer formed a noncovalent base for the insertion of oat husk lignin onto fiber surfaces.

Published
2010