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2. Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks

Author

Daniel Gilbert Weber, Swaantje Casjens, Peter Rozynek, Martin Lehnert, Sandra Zilch-Schöneweis, Oleksandr Bryk, Dirk Taeger, Maria Gomolka, Michaela Kreuzer, Heinz Otten, Beate Pesch, Georg Johnen, and Thomas Brüning

Subjects
Medicine (General), R5-920
Abstract

In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.

Published
2010
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3. Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks

Author

Thomas Brüning, P. Rozynek, Daniel G. Weber, Maria Gomolka, Oleksandr Bryk, Dirk Taeger, Beate Pesch, Swaantje Casjens, Sandra Zilch-Schöneweis, Martin Lehnert, Georg Johnen, Michaela Kreuzer, and Heinz Otten

Subjects
Clinical science, PAXgene, Bioinformatics, Original research, Andrology, blood, microRNA, Medicine, Original Research, marker, Pharmacology, lcsh:R5-920, pre-analytical variation, Messenger RNA, Human blood, business.industry, Biochemistry (medical), RNA, biobank, RNAlater, Molecular Medicine, Cancer gene, RNA stabilization, lcsh:Medicine (General), business
Abstract

In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.

Published
2010
Full Text
View/download PDF

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