1. Direct saliva transcript analysis as a novel non-invasive method for oestrus marker detection in buffaloes.
- Author
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Onteru SK, Baddela VS, Ravinder R, Kaipa O, Nayan V, Singh P, Baithalu RK, and Singh D
- Subjects
- Animals, Biomarkers metabolism, Female, HSP70 Heat-Shock Proteins genetics, Reverse Transcriptase Polymerase Chain Reaction, Toll-Like Receptor 4 genetics, Buffaloes genetics, Estrus genetics, Gene Expression Profiling, Saliva metabolism, Transcription, Genetic genetics
- Abstract
Salivary RNA-based biomarkers are not available for any physiological condition in farm animals. Hence, an objective of this study was to perform salivary transcript analysis in buffaloes. Saliva, after removal of the cells and particulate matter, was directly used for RT-PCR without RNA isolation. Direct saliva transcript analysis (DSTA) showed a suggestively significant higher expression of the Heat shock protein 70 (HSP70) and Toll-like receptor 4 (TLR4) at oestrus than the diestrous period in buffaloes by a non-parametric Mann-Whitney U test. Therefore, DSTA without RNA isolation is an easy method to identify salivary RNA markers for oestrus detection in buffaloes.
- Published
- 2016
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