1. A systematic investigation of the effect of the fluid shear stress on Caco-2 cells towards the optimization of epithelial organ-on-chip models
- Author
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Ludivine C. Delon, Zhaobin Guo, Benjamin Thierry, Anna Oszmiana, Rachel J. Gibson, Clive A. Prestidge, Chia-Chi Chien, Delon, Ludivine C, Guo, Zhaobin, Oszmiana, Anna, Chien, Chia Chi, Gibson, Rachel, Prestidge, Clive, and Thierry, Benjamin
- Subjects
Cell type ,fluid shear stress ,Cell Respiration ,Microfluidics ,microfluidics ,Biophysics ,Bioengineering ,Context (language use) ,02 engineering and technology ,Tight Junctions ,Biomaterials ,intestinal models ,03 medical and health sciences ,Mechanobiology ,Lab-On-A-Chip Devices ,Cytochrome P-450 CYP3A ,Humans ,Cytoskeleton ,Cell Shape ,030304 developmental biology ,0303 health sciences ,Microvilli ,Tight junction ,Chemistry ,Microfilament Proteins ,Epithelial Cells ,021001 nanoscience & nanotechnology ,Intestinal epithelium ,Actins ,Mitochondria ,Mucus ,Vacuolization ,Mechanics of Materials ,Vacuoles ,Ceramics and Composites ,organ-on-chip ,Stress, Mechanical ,Caco-2 Cells ,Energy Metabolism ,Rheology ,0210 nano-technology - Abstract
Epithelial cells experience constant mechanical forces, including fluid shear stress (FSS) on their apical surface.These forces alter both structure and function. While precise recapitulation of the complex mechanobiology of organs remains challenging, better understanding of the effect of mechanical stimuli is necessary towards the development of biorelevant in vitro models. This is especially relevant to organs-on-chip models which allow for fine control of the culture environment. In this study, the effects of the FSS on Caco-2 cell monolayers were systematically determined using a microfluidic device based on Hele-Shaw geometry. This approach allowed fora physiologically relevant range of FSS (from ∼0 to 0.03 dyn/cm2) to be applied to the cells within a single device. Exposure to microfluidic FSS induced significant phenotypical and functional changes in Caco-2 cell monolayers as compared to cells grown in static conditions. The application of FSS significantly altered the production of mucus, expression of tight junctions, vacuolization, organization of cytoskeleton, formation of microvilli, mitochondrial activity and expression of cytochrome P450. In the context of the intestinal epithelium,this detailed understanding of the effects of the FSS will enable the realization of in vitro organs-on-chip models with well-defined and tailored characteristics to a specific purpose, including for drug and nanoparticle absorption studies. The Hele-Shaw approach used in this study could be readily applied to other cell types and adapted for a wide range of physiologically relevant FSS. Refereed/Peer-reviewed
- Published
- 2019