1. Mining for osteogenic surface topographies
- Author
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Bernke J. Papenburg, Dimitrios Stamatialis, Marcel J. T. Reinders, Meint J. de Boer, Mark-Anthony Bray, Jan de Boer, Shantanu Singh, Frits Hulshof, Clemens van Blitterswijk, Yiping Zhao, Huipin Yuan, David J. Logan, Aliaksei Vasilevich, Anne E. Carpenter, Nick R.M. Beijer, Marloes Levers, Natalie Fekete, Marc Hulsman, Biomaterials Science and Technology, RS: MERLN - Cell Biology - Inspired Tissue Engineering (CBITE), CBITE, Division Instructive Biomaterials Eng, RS: MERLN - Complex Tissue Regeneration (CTR), RS: MERLN - Instructive Biomaterials Engineering (IBE), and CTR
- Subjects
0301 basic medicine ,Bone Regeneration ,Micro-fabrication ,02 engineering and technology ,Tissue engineering ,Osteogenesis ,MINERALIZATION ,Cells, Cultured ,Titanium ,Tissue Scaffolds ,High-throughput screening ,Computational modeling ,021001 nanoscience & nanotechnology ,Cell biology ,ATTACHMENT ,DIFFERENTIATION ,Mechanics of Materials ,HISTONE ACETYLATION ,Female ,Rabbits ,Stem cell ,0210 nano-technology ,EXPRESSION ,Materials science ,Surface Properties ,In silico ,Biophysics ,Bioengineering ,HIGH-THROUGHPUT ,Surface topography ,MESENCHYMAL STEM-CELLS ,MECHANISMS ,03 medical and health sciences ,In vivo ,Cell Adhesion ,Animals ,Humans ,Computer Simulation ,Cell adhesion ,Bone regeneration ,BIOMATERIALS ,Cell Proliferation ,Mechanical Phenomena ,Tissue Engineering ,Cell growth ,Bone implants ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,IMPLANTS ,Nanostructures ,030104 developmental biology ,Bone Substitutes ,2023 OA procedure ,Ceramics and Composites ,Biomedical engineering - Abstract
Stem cells respond to the physicochemical parameters of the substrate on which they grow. Quantitative material activity relationships - the relationships between substrate parameters and the phenotypes they induce - have so far poorly predicted the success of bioactive implant surfaces. In this report, we screened a library of randomly selected designed surface topographies for those inducing osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Cell shape features, surface design parameters, and osteogenic marker expression were strongly correlated in vitro. Furthermore, the surfaces with the highest osteogenic potential in vitro also demonstrated their osteogenic effect in vivo: these indeed strongly enhanced bone bonding in a rabbit femur model. Our work shows that by giving stem cells specific physicochemical parameters through designed surface topographies, differentiation of these cells can be dictated. (C) 2017 Elsevier Ltd. All rights reserved.
- Published
- 2017
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