1. A dual-modal PET/near infrared fluorescent nanotag for long-term immune cell tracking
- Author
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Naga Vara Kishore Pillarsetty, Mezruh Turkekul, Yiyu Dong, Stefan Harmsen, Richard Ting, Jose Lobo, Emin Ilker Medine, Omer Aras, Maxim A. Moroz, Oguz Akin, Fuad Nurili, and Vladimir Ponomarev
- Subjects
Biodistribution ,Adoptive cell transfer ,Biophysics ,Bioengineering ,02 engineering and technology ,Immunotherapy, Adoptive ,Article ,Biomaterials ,03 medical and health sciences ,Immune system ,Cell Line, Tumor ,medicine ,Cytotoxic T cell ,Humans ,Tissue Distribution ,Viability assay ,030304 developmental biology ,0303 health sciences ,Chemistry ,Cancer ,021001 nanoscience & nanotechnology ,medicine.disease ,Imaging agent ,Chimeric antigen receptor ,Mechanics of Materials ,Cell Tracking ,Positron-Emission Tomography ,Ceramics and Composites ,Cancer research ,0210 nano-technology - Abstract
Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticle-based imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.
- Published
- 2019