1. In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium
- Author
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Dionne Tannetta, Michiya Matsusaki, Paul Verkade, Simon Grant, John D. Aplin, Gavin Collett, Jon Hanley, Aman Sood, Fiona Day, Nagaraj D. Halemani, Catherine Murdoch-Davis, Akihiro Nishiguchi, Jennifer McGarvey, Ian L. Sargent, Helena Kemp, Mitsuru Akashi, Catherine E. Gilmore, and C. Patrick Case
- Subjects
Endothelium ,Placenta ,Biophysics ,Connective tissue ,Bioengineering ,02 engineering and technology ,Biology ,Biomaterials ,03 medical and health sciences ,Pregnancy ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,Secretion ,Cells, Cultured ,reproductive and urinary physiology ,Connective Tissue Cells ,030304 developmental biology ,Neurons ,0303 health sciences ,Fetus ,Trophoblast ,021001 nanoscience & nanotechnology ,Embryonic stem cell ,In vitro ,Trophoblasts ,Cell biology ,medicine.anatomical_structure ,Mechanics of Materials ,embryonic structures ,Ceramics and Composites ,Female ,Endothelium, Vascular ,0210 nano-technology - Abstract
Fetal development may be compromised by adverse events at the placental interface between mother and fetus. However, it is still unclear how the communication between mother and fetus occurs through the placenta. In vitro - models of the human placental barrier, which could help our understanding and which recreate three-dimensional (3D) structures with biological functionalities and vasculatures, have not been reported yet. Here we present a 3D-vascularized human primary placental barrier model which can be constructed in 1 day. We illustrate the similarity of our model to first trimester human placenta, both in its structure and in its ability to respond to altered oxygen and to secrete factors that cause damage cells across the barrier including embryonic cortical neurons. We use this model to highlight the possibility that both the trophoblast and the endothelium within the placenta might play a role in the fetomaternal dialogue.
- Published
- 2019
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