1. Engineering discoidal polymeric nanoconstructs with enhanced magneto-optical properties for tumor imaging.
- Author
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Key J, Aryal S, Gentile F, Ananta JS, Zhong M, Landis MD, and Decuzzi P
- Subjects
- Animals, Cell Death drug effects, HeLa Cells, Humans, Mice, Mice, SCID, Microscopy, Fluorescence, Nanoparticles toxicity, Nanoparticles ultrastructure, Polylactic Acid-Polyglycolic Acid Copolymer, Rhodamines metabolism, Diagnostic Imaging methods, Lactic Acid chemistry, Magnetic Phenomena, Methacrylates chemistry, Nanotechnology methods, Neoplasms diagnosis, Optical Phenomena, Polyethylene Glycols chemistry, Polyglycolic Acid chemistry
- Abstract
The in vivo performance of nanoparticles is affected by their size, shape and surface properties. Fabrication methods based on emulsification and nano-precipitation cannot control these features precisely and independently over multiple scales. Herein, discoidal polymeric nanoconstructs (DPNs) with a diameter of 1000 nm and a height of 500 nm are demonstrated via a modified hydrogel-template strategy. The DPNs are obtained by mixing in one synthesis step the constituent polymers - poly(lactic acid-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) dimethacrylate - and the payload with magneto-optical properties - 5 nm ultra-small super-paramagnetic iron oxide nanoparticles (SPIOs) and Rhodamine B dye (RhB). The DPN geometrical features are characterized by multiple microscopy techniques. The release of the Rhodamine B dye is pH dependent and increases under acidic conditions by the enhanced hydrolysis of the polymeric matrix. Each DPN is loaded with ~100 fg of iron and can be efficiently dragged by static and external magnetic fields. Moreover, the USPIO confinement within the DPN porous structure is responsible for a significant enhancement in MRI relaxivity (r2 ~ 500 (mMs)(-1)), up to ~5 times larger than commercially available systems. These nanoconstructs suggest a general strategy to engineer theranostic systems for anti-angiogenic treatment and vascular imaging., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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