1. A polyethylenimine-based diazeniumdiolate nitric oxide donor accelerates wound healing
- Author
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Keyu Tang, Run Lin, Chuwei Liu, Su Zhou, Yan Zhang, Bin Chen, Tao Zhang, Weiling He, Jianyong Yang, and Nan Li
- Subjects
Male ,Biocompatibility ,Cell Survival ,Biomedical Engineering ,macromolecular substances ,02 engineering and technology ,Polyethylene glycol ,010402 general chemistry ,Nitric Oxide ,01 natural sciences ,Nitric oxide ,Cell Line ,chemistry.chemical_compound ,Mice ,Structure-Activity Relationship ,PEG ratio ,medicine ,Animals ,Polyethyleneimine ,General Materials Science ,Nitric Oxide Donors ,Polyethylenimine ,Mice, Inbred BALB C ,Wound Healing ,Dose-Response Relationship, Drug ,Molecular Structure ,technology, industry, and agriculture ,Granulation tissue ,021001 nanoscience & nanotechnology ,Controlled release ,0104 chemical sciences ,medicine.anatomical_structure ,chemistry ,0210 nano-technology ,Wound healing ,Azo Compounds ,Nuclear chemistry - Abstract
Nitric oxide (NO) plays a pivotal role in the cutaneous healing process and a topical supplement of NO is beneficial for wound repair. In this work, a novel polyethylenimine (PEI) based diazeniumdiolate nitric oxide donor was prepared. The obtained polymer (PEI-PO-NONOate) was characterized by Fourier transform infrared (FTIR) spectroscopy, UV-vis absorption spectra, and nuclear magnetic resonance (NMR). The PEI-PO-NONOate polymer was stable under anhydrous conditions at different temperatures. A total of 0.57 μmol gaseous NO was released from 1.0 mg of the PEI-PO-NONOate polymer in PBS of pH 7.4 and it presented a proton-driven release pattern. Furthermore, the PEI-PO-NONOate polymer exhibited a controlled release profile sustained for over 30 hours within the polyethylene glycol (PEG) mixture system. Cytotoxicity evaluation was performed on L929 cells. Full-thickness excisional cutaneous wound models of mice were prepared and the PEI-PO-NONOate polymer was applied to investigate its effects on wound healing. The results revealed that the PEI-PO-NONOates exhibited good biocompatibility. It was also found that the PEI-PO-NONOate polymer promoted cutaneous wound healing and closure with enhanced granulation tissue formation, collagen deposition, and angiogenesis, as compared to the control. In summary, a novel nitric oxide releasing polymer with high loading efficiency and a controlled release profile was developed which presented the potential for application in the acceleration of cutaneous wound healing.
- Published
- 2019