16 results on '"WEI GAO"'
Search Results
2. Paraquat Exposure Induces Pulmonary Cell Mitophagy by Enhancing the PINK1/Parkin Signaling
- Author
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Xisheng Xie, Zhipeng Zhan, Wei Gao, Jie Feng, and Kaixiang Liu
- Subjects
A549 cell ,General Immunology and Microbiology ,Article Subject ,Chemistry ,PINK1 ,General Medicine ,Lung injury ,respiratory system ,medicine.disease ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Parkin ,Cell biology ,nervous system diseases ,respiratory tract diseases ,Fibrosis ,Pulmonary fibrosis ,Mitophagy ,medicine ,Medicine ,Oxidative stress - Abstract
Paraquat (PQ) poisoning can cause oxidative stress, acute lung injury (ALI), and fibrosis. Excess oxidative stress can induce mitophagy. However, whether PQ exposure can induce mitophagy, contributing to the development of ALI and pulmonary fibrosis in vivo has not been clarified. Here, we show that PQ exposure induces ALI and fiber accumulation in the lung of rats in a time-dependent manner, accompanied by upregulating fibronectin (FN) and Collagen I (COL-I) expression. PQ exposure increased mitophagosome formation and PINK1 and Parkin expression in the lungs of rats. Similarly, PQ exposure reduced the viability and mitochondrial membrane potential, but enhanced FN, COL-I, PINK1, and Parkin expression in A549 cells. In contrast, PINK1 silencing significantly mitigated the PQ-upregulated Parkin, FN, and COL-I expression in A549 cells. Hence, PQ exposure induced ALI and fibrosis in rats by enhancing the PINK1/Parkin signaling and profibrotic factor expression in the lungs. Therefore, our findings suggest that the PINK1/Parkin signaling may be new therapeutic targets and may provide new insights in the pathogenesis of PQ-related ALI in rats.
- Published
- 2020
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3. Predicting Gram-Positive Bacterial Protein Subcellular Location by Using Combined Features
- Author
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Xiao-Wei Gao and Feng-Min Li
- Subjects
0301 basic medicine ,Support Vector Machine ,Article Subject ,Improved method ,Computational biology ,Biology ,Gram-Positive Bacteria ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Bacterial protein ,03 medical and health sciences ,0302 clinical medicine ,Bacterial Proteins ,Humans ,Protein function prediction ,Databases, Protein ,Gram ,chemistry.chemical_classification ,General Immunology and Microbiology ,Computational Biology ,General Medicine ,biology.organism_classification ,Amino acid ,Support vector machine ,Dipeptide composition ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Medicine ,Bacteria ,Research Article - Abstract
There are a lot of bacteria in the environment, and Gram-positive bacteria are the most common ones. Some Gram-positive bacteria are very harmful to the human body, so it is significant to predict Gram-positive bacterial protein subcellular location. And identification of Gram-positive bacterial protein subcellular location is important for developing effective drugs. In this paper, a new Gram-positive bacterial protein subcellular location dataset was established. The amino acid composition, the gene ontology annotation information, the hydropathy dipeptide composition information, the amino acid dipeptide composition information, and the autocovariance average chemical shift information were selected as characteristic parameters, then these parameters were combined. The locations of Gram-positive bacterial proteins were predicted by the Support Vector Machine (SVM) algorithm, and the overall accuracy (OA) reached 86.1% under the Jackknife test. The overall accuracy (OA) in our predictive model was higher than those in existing methods. This improved method may be helpful for protein function prediction.
- Published
- 2020
4. Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome
- Author
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Chang-chun Guo, Ying-nan Ju, Dong-mei Li, Xue Lin, and Wei Gao
- Subjects
Lipopolysaccharides ,Male ,Lipopolysaccharide ,Article Subject ,Neutrophils ,Ventilator-Induced Lung Injury ,Interleukin-1beta ,lcsh:Medicine ,Lung injury ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Desflurane ,chemistry.chemical_compound ,0302 clinical medicine ,030202 anesthesiology ,medicine ,Tidal Volume ,Animals ,Rats, Wistar ,Lung ,Tidal volume ,Respiratory Distress Syndrome ,General Immunology and Microbiology ,Isoflurane ,business.industry ,Tumor Necrosis Factor-alpha ,Macrophages ,lcsh:R ,NF-kappa B ,General Medicine ,respiratory system ,Respiration, Artificial ,Interleukin-10 ,Rats ,respiratory tract diseases ,medicine.anatomical_structure ,030228 respiratory system ,chemistry ,Anesthesia ,Breathing ,Tumor necrosis factor alpha ,business ,Corrigendum ,medicine.drug ,Research Article - Abstract
Ventilator-induced lung injury aggravates the existing lung injury. This study investigated the effect of desflurane on VILI in a rat model of acute respiratory distress syndrome. Forty-eight rats were randomized into a sham (S) group, control (C) group, lipopolysaccharide/ventilation (LV) group, lipopolysaccharide/ventilation/desflurane (LVD) group, or lipopolysaccharide/low ventilation with and without desflurane (LLV and LLVD) groups. Rats in the S group received anesthesia only. Rats in the LV and LVD groups received lipopolysaccharide and were ventilated with a high tidal volume. Rats in LLV and LLVD groups were treated as the LV and LVD groups and ventilated with a low tidal volume. PaO2/FiO2, lung wet-to-dry weight ratios, concentrations of inflammatory factors in serum and BALF, histopathologic analysis of lung tissue, and levels of nuclear factor- (NF-) κB protein in lung tissue were investigated. PaO2/FiO2 was significantly increased by desflurane. Total cell count, macrophages, and neutrophils in BALF and proinflammatory factors in BALF and serum were significantly decreased by desflurane, while IL-10 was increased. The histopathological changes and levels of NF-κB protein in lung tissue were decreased by desflurane. The results indicated that desflurane ameliorated VILI in a rat model of acute respiratory distress syndrome.
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- 2018
- Full Text
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5. Solid Pseudopapillary Neoplasm of the Pancreas: Clinicopathologic Feature, Risk Factors of Malignancy, and Survival Analysis of 53 Cases from a Single Center
- Author
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He Song, Weiwei Sheng, Wei Gao, Banghua Zhong, Jianping Zhou, and Ming Dong
- Subjects
Adult ,Male ,Abdominal pain ,medicine.medical_specialty ,Adolescent ,Article Subject ,medicine.medical_treatment ,lcsh:Medicine ,Malignancy ,Single Center ,Disease-Free Survival ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Pancreatectomy ,0302 clinical medicine ,Risk Factors ,medicine ,Carcinoma ,Humans ,Neoplasm ,Pancreas ,Survival analysis ,Aged ,General Immunology and Microbiology ,business.industry ,lcsh:R ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Carcinoma, Papillary ,Surgery ,Pancreatic Neoplasms ,Treatment Outcome ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,030211 gastroenterology & hepatology ,medicine.symptom ,Tomography, X-Ray Computed ,business ,Research Article - Abstract
Introduction. Solid pseudopapillary neoplasm (SPN) of the pancreas is a rare tumor of low malignant potential. The aim of this study was designed to evaluate the clinicopathologic feature, predictive factors of malignancy, and survival from experience of a single center. Methods. 53 consecutive patients who underwent surgery for a pathologically definitive SPN were retrospectively reviewed. Results. A total of 53 cases included 7 male cases and 46 female cases with the median age of 35.4 years (14–67). Abdominal pain and mass were the most common clinical presentations. The radiological presentations were consistent with solid and cystic pattern in 18 cases, solid pattern in 25 cases, and cystic pattern in 10 cases. The predominant location of tumor was pancreatic body and tail. The mean size of the tumors was 6.4 cm. Aggressive en bloc resection combined with organ-preserving should be indicated whenever feasible. Follow-up information was available for 48 patients with a median follow-up time of 48 months. The 5-year disease-specific survival was 95.7%. Incomplete capsule was not only the predictive factor of malignancy but also the significant predictor of disease-specific survival. Conclusion. Incomplete capsule may suggest a malignant SPN and a prognostic indicator of disease-specific survival. We recommend that surgeons consider a more radical resection with an incomplete capsule of tumor.
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- 2017
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6. The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse
- Author
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Jing-Yin Dong, Jin-Xiao Liang, Chi Ma, Wei Gao, and Qiu Yan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,endocrine system ,Article Subject ,lcsh:Medicine ,Apoptosis ,Radiation-Protective Agents ,Ovary ,Primary Ovarian Insufficiency ,Biology ,Endometrium ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Acetylcysteine ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Ovarian Follicle ,Radiation, Ionizing ,Internal medicine ,medicine ,Animals ,Humans ,Embryo Implantation ,Ovarian follicle ,Ovarian Reserve ,Ovarian reserve ,Cell Proliferation ,General Immunology and Microbiology ,lcsh:R ,General Medicine ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Corrigendum ,Oxidative stress ,Research Article ,medicine.drug ,Hormone - Abstract
Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation. In addition, morphological observations on the endometrial luminal epithelium and the fertility assessment were performed. We found that NAC successfully restored the ovarian and uterine function, enhanced the embryo implantation, improved the follicle development, and altered the abnormal hormone levels through reducing the oxidative stress and apoptosis level in granulosa cells while promoting the proliferation of granulosa cells. In conclusion, the radioprotective effect of NAC on mice ovary from X-irradiation was assessed, and our results suggested that NAC can be a potential radioprotector which is capable of preventing the ovarian failure occurrence and restoring the ovarian reserve.
- Published
- 2017
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7. The Roles of CD73 in Cancer
- Author
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Ke Dong, Zhao-wei Gao, and Huizhong Zhang
- Subjects
Adenosine ,Angiogenesis ,Carcinogenesis ,lcsh:Medicine ,Review Article ,Biology ,GPI-Linked Proteins ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Neovascularization ,Mice ,Immune system ,Neoplasms ,medicine ,Animals ,Humans ,5'-Nucleotidase ,Cell Proliferation ,Regulation of gene expression ,General Immunology and Microbiology ,Neovascularization, Pathologic ,lcsh:R ,Cancer ,General Medicine ,Purinergic signalling ,medicine.disease ,Adenosine Monophosphate ,Gene Expression Regulation, Neoplastic ,Cancer cell ,Immunology ,Cancer research ,medicine.symptom - Abstract
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of cancer. Various factors and mechanisms are employed to regulate expression of CD73. Accumulating studies have shown that CD73 is a key regulatory molecule of cancer cells proliferation, migration and invasionin vitro, tumor angiogenesis, and tumor immune escapein vivo. With such important roles in cancer, CD73 has become an appealing therapy target. Recent evidences in mice models demonstrated that targeted blockade of CD73 could be a favorable therapeutic approach for cancer patients in the future. In this review, we will summarize the multiple roles of CD73 in cancer development, including its clinical significance, its promotive effects on tumor growth, metastasis, and angiogenesis, and its suppressive effects on immune response, regulatory mechanisms of CD73 expression, and current situation of anti-CD73 cancer therapy.
- Published
- 2014
8. Transcription Regulation of E-Cadherin by Zinc Finger E-Box Binding Homeobox Proteins in Solid Tumors
- Author
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Thian-Sze Wong, Wei Gao, and Jimmy Yu-Wai Chan
- Subjects
Protein family ,Transcription, Genetic ,lcsh:Medicine ,Review Article ,Biology ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Neoplasms ,Transcriptional regulation ,medicine ,Humans ,Enhancer ,Zinc finger ,Homeodomain Proteins ,General Immunology and Microbiology ,Cadherin ,lcsh:R ,Zinc Fingers ,General Medicine ,Cadherins ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Regulatory sequence ,Cancer research ,Homeobox ,Carcinogenesis - Abstract
Downregulation of E-cadherin in solid tumors with regional migration and systematic metastasis is well recognized. In view of its significance in tumorigenesis and solid cancer progression, studies on the regulatory mechanisms are important for the development of target treatment and prediction of clinical behavior for cancer patients. The vertebrate zinc finger E-box binding homeobox (ZEB) protein family comprises 2 major members: ZEB1 and ZEB2. Both contain the motif for specific binding to multiple enhancer boxes (E-boxes) located within the short-range transcription regulatory regions of the E-cadherin gene. Binding of ZEB1 and ZEB2 to the spaced E-cadherin E-boxes has been implicated in the regulation of E-cadherin expression in multiple human cancers. The widespread functions of ZEB proteins in human malignancies indicate their significance. Given the significance of E-cadherin in the solid tumors, a deeper understanding of the functional role of ZEB proteins in solid tumors could provide insights in the design of target therapy against the migratory nature of solid cancers.
- Published
- 2014
9. Differential Expression of Long Noncoding RNA in Primary and Recurrent Nasopharyngeal Carcinoma
- Author
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Thian-Sze Wong, Wei Gao, and Jimmy Yu Wai Chan
- Subjects
Adult ,Male ,Microarray ,Adolescent ,Article Subject ,Nasopharyngeal neoplasm ,lcsh:Medicine ,Biology ,Bioinformatics ,General Biochemistry, Genetics and Molecular Biology ,Pathogenesis ,Young Adult ,medicine ,otorhinolaryngologic diseases ,Humans ,Child ,Aged ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Nasopharyngeal Carcinoma ,General Immunology and Microbiology ,Microarray analysis techniques ,Gene Expression Profiling ,Carcinoma ,lcsh:R ,Nasopharyngeal Neoplasms ,General Medicine ,Middle Aged ,medicine.disease ,Long non-coding RNA ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,Nasopharyngeal carcinoma ,ROC Curve ,Cancer research ,Female ,RNA, Long Noncoding ,Neoplasm Recurrence, Local ,Research Article - Abstract
Background. Recent studies suggested that non-protein-coding genes are implicated in the tumorigenic process of nasopharyngeal carcinoma (NPC). In the present study, we aimed to identify the differentially expressed long noncoding RNA (lncRNA) using data available in the public domain. Methods. Microarray data set GSE12452 was reannotated with ncFANs. Real-time quantitative PCR was used to quantify and validate the identified lncRNAs in NPC. Results. In primary NPC, upregulation of lnc-C22orf32-1, lnc-AL355149.1-1, and lnc-ZNF674-1 was observed. High levels of lnc-C22orf32-1 and lnc-AL355149.1-1 were significantly associated with the male patients. In addition, increased expression of lnc-C22orf32-1 and lnc-ZNF674-1 was associated with advanced tumor stages. Recurrent NPC displayed a distinctive lncRNA expression pattern. lnc-BCL2L11-3 was significantly increased in the recurrent NPC tissues. In addition, significant reduction of lnc-AL355149.1-1 and lnc-ZNF674-1 was observed in the recurrent NPC tissues. Conclusions. Our results demonstrated that it is feasible to identify the differentially expressed lncRNA in the microarray dataset by functional reannotation. The association of lncRNA with gender and tumor size implicated that lncRNA possibly plays a part in the pathogenesis of primary NPC. Further, the distinctive lncRNA identified in the recurrent NPC may reveal a distinctive development mechanism underlying tumor recurrence. © 2014 Wei Gao et al., published_or_final_version
- Published
- 2014
10. Endostatin/Collagen XVIII Is Increased in Cerebrospinal Fluid after Severe Traumatic Brain Injury
- Author
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Shiming Ju, Li-Xia Xue, Wen-Wei Gao, Shiwen Chen, Heng-Li Tian, Yan Guo, Heli Cao, and Hao Chen
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Article Subject ,Traumatic brain injury ,lcsh:Medicine ,Enzyme-Linked Immunosorbent Assay ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,Cerebrospinal fluid ,Internal medicine ,medicine ,Humans ,Collagen XVIII ,Clinical significance ,Glasgow Coma Scale ,Aged ,General Immunology and Microbiology ,Receiver operating characteristic ,business.industry ,lcsh:R ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Angiogenesis inhibitor ,Collagen Type XVIII ,ROC Curve ,Brain Injuries ,Clinical Study ,Female ,Endostatin ,business - Abstract
Recent studies have suggested that endogenous angiogenesis inhibitor endostatin/collagen XVIII might play an important role in the secondary brain injury following traumatic brain injury (TBI). In this study, we measured endostatin/collagen XVIII concentrations serially for 1 week after hospitalization by using the enzyme-linked immunosorbent assay method in the cerebrospinal fluid (CSF) of 30 patients with TBI and a Glasgow Coma Scale (GCS) score of 8 or less on admission. There was a significant trend toward increased CSF levels of endostatin after TBI versus control from 72 h after injury. In patients with GCS score of 3–5, CSF endostatin concentration was substantially higher at 72 h after injury than that in patients with GCS score of 6–8 (P<0.05) and peaked rapidly at day 5 after injury, but decreased thereafter. The CSF endostatin concentration in 12 patients with an unfavorable outcome was significantly higher than that in 18 patients with a favorable outcome at day 5 (P=0.043) and day 7 (P=0.005) after trauma. Receiver operating characteristic curve analysis suggested a reliable operating point for the 7-day CSF endostatin concentration predicting poor prognosis to be 67.29 pg/mL. Our preliminary findings provide new evidence that endostatin/collagen XVIII concentration in the CSF increases substantially in patients with sTBI. Its dynamic change may have some clinical significance on the judgment of brain injury severity and the assessment of prognosis. This trial is registered with the ClinicalTrials.gov Identifier:NCT01846546.
- Published
- 2013
11. Pentraxin-3 Predicts Long-Term Cardiac Events in Patients with Chronic Heart Failure.
- Author
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Haibo Liu, Xiaofang Guo, Kang Yao, Chunming Wang, Guozhong Chen, Wei Gao, Jie Yuan, Wangun Yu, and Junbo Ge
- Subjects
HEART failure risk factors ,HEART failure ,BLOOD proteins ,CONFIDENCE intervals ,LONGITUDINAL method ,PROPORTIONAL hazards models ,KAPLAN-Meier estimator ,INDEPENDENT variables ,ODDS ratio ,PROGNOSIS - Abstract
The aim of this study was to investigate the long-term prognostic value of pentraxin-3 (PTX3) in patients with chronic heart failure (CHF). 377 patients were prospectively followed up for 3 years to determine cardiac events including cardiac death or rehospitalization for worsening heart failure. The plasma PTX3 levels were significantly higher in CHF patients than in healthy subjects (p < 0.001), and they increased with advancing New York Heart Association (NYHA) Functional Classification (p < 0.001). Plasma PTX3 levels in CHF patients with cardiac events were significantly higher than in event-free patients (p < 0.001). We determined the normal upper limit of plasma PTX3 levels from the mean + 2 SD value of 64 control subjects (3.64 ng/mL). A Kaplan-Meier analysis revealed that patients with increased PTX3 (>3.64 ng/mL) were at a higher risk for cardiac events than those without increased PTX3 (p < 0.01). A multifactorial Cox proportional hazards model showed that increased PTX3 (≥3.64 nglmL) was an independent risk factor for cardiac events in CHF patients (hazard ratio (HR) = 4.224, p < 0.01; 95% CI: 1.130-15.783). Plasma PTX3 levels are a long-term independent predictor of prognosis in patients with CHF. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
12. The Roles of CD73 in Cancer.
- Author
-
Zhao-wei Gao, Ke Dong, and Hui-zhong Zhang
- Abstract
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of cancer. Various factors and mechanisms are employed to regulate expression of CD73. Accumulating studies have shown that CD73 is a key regulatory molecule of cancer cells proliferation, migration and invasion in vitro, tumor angiogenesis, and tumor immune escape in vivo. With such important roles in cancer, CD73 has become an appealing therapy target. Recent evidences in micemodels demonstrated that targeted blockade of CD73 could be a favorable therapeutic approach for cancer patients in the future. In this review, we will summarize the multiple roles of CD73 in cancer development, including its clinical significance, its promotive effects on tumor growth, metastasis, and angiogenesis, and its suppressive effects on immune response, regulatory mechanisms of CD73 expression, and current situation of anti-CD73 cancer therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
13. Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma.
- Author
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Wei Gao, Jimmy Yu-Wai Chan, and Thian-Sze Wong
- Abstract
Background. The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC). Methods. Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs. Results. Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells. Conclusions. Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
14. Differential Expression of Long Noncoding RNA in Primary and Recurrent Nasopharyngeal Carcinoma.
- Author
-
Wei Gao, Jimmy Yu-Wai Chan, and Thian-Sze Wong
- Abstract
Background. Recent studies suggested that non-protein-coding genes are implicated in the tumorigenic process of nasopharyngeal carcinoma (NPC). In the present study, we aimed to identify the differentially expressed long noncoding RNA (lncRNA) using data available in the public domain. Methods. Microarray data set GSE12452 was reannotated with ncFANs. Real-time quantitative PCR was used to quantify and validate the identified lncRNAs in NPC. Results. In primary NPC, upregulation of lnc-C22orf32-1, lnc- AL355149.1-1, and lnc-ZNF674-1 was observed. High levels of lnc-C22orf32-1 and lnc-AL355149.1-1 were significantly associated with the male patients. In addition, increased expression of lnc-C22orf32-1 and lnc-ZNF674-1 was associated with advanced tumor stages. Recurrent NPC displayed a distinctive lncRNA expression pattern. lnc-BCL2L11-3 was significantly increased in the recurrent NPC tissues. In addition, significant reduction of lnc-AL355149.1-1 and lnc-ZNF674-1 was observed in the recurrent NPC tissues. Conclusions. Our results demonstrated that it is feasible to identify the differentially expressed lncRNA in the microarray dataset by functional reannotation. The association of lncRNA with gender and tumor size implicated that lncRNA possibly plays a part in the pathogenesis of primary NPC. Further, the distinctive lncRNA identified in the recurrent NPC may reveal a distinctive development mechanism underlying tumor recurrence. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
15. Interactions between E-Cadherin and MicroRNA Deregulation in Head and Neck Cancers: The Potential Interplay.
- Author
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Thian-Sze Wong, Wei Gao, and Jimmy Yu-Wai Chan
- Abstract
E-cadherin expression in the head and neck epithelium is essential for the morphogenesis and homeostasis of epithelial tissues. The cadherin-mediated cell-cell contacts are required for the anchorage-dependent growth of epithelial cells. Further, survival and proliferation require physical tethering created by proper cell-cell adhesion. Otherwise, the squamous epithelial cells will undergo programmed cell death. Head and neck cancers can escape from anoikis and enter into the epithelial-mesenchymal transition stages via the modulation of E-cadherin expression with epigenetic mechanisms. At epigenetic level, gene expression control is not dependent on the DNA sequence. In the context of E-cadherin regulation in head and neck cancers, 2 major mechanisms including de novo promoter hypermethylation and microRNA dysregulation are most extensively studied. Both of them control E-cadherin expression at transcription level and subsequently hinder the overall E-cadherin protein level in the head and neck cancer cells. Increasing evidence suggested that microRNA mediated E-cadherin expression in the head and neck cancers by directly/indirectly targeting the transcription suppressors of E-cadherin, ZEB1 and ZEB2. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
16. Endostatin/Collagen XVIII Is Increased in Cerebrospinal Fluid after Severe Traumatic Brain Injury.
- Author
-
Hao Chen, Li-Xia Xue, He-Li Cao, Shi-Wen Chen, Yan Guo, Wen-Wei Gao, Shi-Ming Ju, and Heng-Li Tian
- Abstract
Recent studies have suggested that endogenous angiogenesis inhibitor endostatin/collagen XVIII might play an important role in the secondary brain injury following traumatic brain injury (TBI). In this study, we measured endostatin/collagen XVIII concentrations serially for 1 week after hospitalization by using the enzyme-linked immunosorbent assay method in the cerebrospinal fluid (CSF) of 30 patients with TBI and a Glasgow Coma Scale (GCS) score of 8 or less on admission. There was a significant trend toward increased CSF levels of endostatin after TBI versus control from 72 h after injury. In patients with GCS score of 3-5, CSF endostatin concentration was substantially higher at 72 h after injury than that in patients with GCS score of 6-8 (P < 0.05) and peaked rapidly at day 5 after injury, but decreased thereafter. The CSF endostatin concentration in 12 patients with an unfavorable outcome was significantly higher than that in 18 patients with a favorable outcome at day 5 (P = 0.043) and day 7 (P = 0.005) after trauma. Receiver operating characteristic curve analysis suggested a reliable operating point for the 7-day CSF endostatin concentration predicting poor prognosis to be 67.29 pg/mL. Our preliminary findings provide new evidence that endostatin/collagen XVIII concentration in the CSF increases substantially in patients with sTBI. Its dynamic change may have some clinical significance on the judgment of brain injury severity and the assessment of prognosis. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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