1. Massage of Bladder Meridian Relieved Anxiety Induced by Chronic Stress in Rats.
- Author
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Lu, Ping, Fang, Min, Yao, Lei, Zhang, Nan, Xu, Ke, and He, Pei
- Subjects
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PROTEIN analysis , *ANXIETY treatment , *BIOLOGICAL models , *CYTOKINES , *ANIMAL behavior , *STATISTICS , *INTERLEUKINS , *MASSAGE therapy , *BLADDER , *HIPPOCAMPUS (Brain) , *STAINS & staining (Microscopy) , *ANALYSIS of variance , *CHRONIC diseases , *ANIMAL experimentation , *INFLAMMATION , *SERUM , *WESTERN immunoblotting , *AUTOPHAGY , *IMMUNOHISTOCHEMISTRY , *QI (Chinese philosophy) , *SUPEROXIDE dismutase , *NEUROTRANSMITTERS , *RATS , *MALONDIALDEHYDE , *ACUPUNCTURE points , *ENZYME-linked immunosorbent assay , *MENTAL depression , *DATA analysis , *DATA analysis software , *PSYCHOLOGICAL stress - Abstract
The aim of this paper was to explore the mechanism of bladder meridian massage (BMM) on anxiety in rats with chronic stress. Chronic stress induced rats to establish rat anxiety model. The sugar water preference (SPF), tail suspension time (TST), and forced swimming time (FST) of rats were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), and inflammatory cytokines in serum and hippocampus of rats were detected. Brain neurotransmitters (dopamine (DA), 5- hydroxytryptamine (5-HT), and norepinephrine (NE)) were detected by enzyme-linked immunosorbent assay (ELISA) kits. Immunohistochemistry and western blotting were used to detect autophagy protein expression in hippocampus of rats. BMM significantly increased SPF, decreased TST and FST, increased SOD level in serum and hippocampus, and decreased MDA level and cytokine level. BMM reversed the changes of neurotransmitters. At the same time, BMM significantly decreased autophagy protein expression in hippocampus of rats. The above results show that BMM significantly relieve anxiety induced by chronic stress in rats. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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