15 results on '"Lei, Lei"'
Search Results
2. Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy
- Author
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Ruizhen Sun, Lei Lei, Tian-tian Gong, Qi Wang, Ying Fan, Zhiyan Shan, Xiao Liang, and Hui Liu
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0301 basic medicine ,Article Subject ,Macrophage polarization ,lcsh:Medicine ,Nitric Oxide Synthase Type II ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,Autophagy ,Animals ,Macrophage ,S1PR1 ,Foam cell ,Sirolimus ,S1PR3 ,Membrane Glycoproteins ,S1PR5 ,Arginase ,General Immunology and Microbiology ,Fingolimod Hydrochloride ,Interleukin-6 ,Chemistry ,lcsh:R ,General Medicine ,Transforming Growth Factor alpha ,Atherosclerosis ,Cell biology ,Lipoproteins, LDL ,Receptors, Lysosphingolipid ,030104 developmental biology ,Cyclooxygenase 2 ,Macrophages, Peritoneal ,Microtubule-Associated Proteins ,Biomarkers ,Mannose receptor ,Foam Cells ,Research Article - Abstract
Foam cell formation and macrophage polarization are involved in the pathologic development of atherosclerosis, one of the most important human diseases affecting large and medium artery walls. This study was designed to assess the effects of rapamycin and FTY720 (fingolimod) on macrophages and foam cells. Mouse peritoneal macrophages were collected and treated with rapamycin and FTY720 to study autophagy, polarization, and lipid accumulation. Next, foam cells were formed by oxidizing low-density lipoprotein to observe changes in lipid accumulation, autophagy, and polarization in rapamycin-treated or FTY720-treated foam cells. Lastly, foam cells that had been treated with rapamycin and FTY720 were evaluated for sphingosine 1-phosphate receptor (S1prs) expression. Autophagy microtubule-associated protein 1 light chain 3- (LC3-) II was increased, and classically activated macrophage phenotype markers interleukin- (IL-) 6, cyclooxygenase-2 (COX2), and inducible nitric oxide synthase (iNOS) were increased, whereas alternatively activated macrophage phenotype markers transforming growth factor- (TGF-)β, arginase 1 (Arg1), and mannose receptor C-type 1 (Mrc1) were decreased by rapamycin in peritoneal macrophages. LC3-II was also obviously enhanced, though polarization markers were unchanged in rapamycin-treated foam cells. Moreover, lipid accumulation was inhibited in rapamycin-treated macrophage cells but was unchanged in rapamycin-treated foam cells. For FTY720, LC3-II did not change, whereas TGF-β, Arg1 and Mrc1 were augmented, and IL-6 was suppressed in macrophages. However, LC3-II was increased, and TGF-β, ARG1 and MRC1 were strikingly augmented, whereas IL-6, COX2 and iNOS could be suppressed in foam cells. Furthermore, lipid accumulation was alleviated in FTY720-treated foam cells. Additionally, S1pr1 was markedly decreased in foam cells (P< .05); S1pr2, S1pr3, S1pr4 and S1pr5 were unchanged in rapamycin-treated foam cells. In FTY720-treated foam cells, S1pr3 and S1pr4 were decreased, and S1pr1, S1pr2 and S1pr5 were unchanged. Therefore, we deduced that rapamycin stimulated classically activated macrophages and supressed early atherosclerosis. Rapamycin may also stabilize artery plaques by preventing apoptosis and S1PR1 in advanced atherosclerosis. FTY720 allowed transformation of foam cells into alternatively activated macrophages through the autophagy pathway to alleviate advanced atherosclerosis.
- Published
- 2018
3. EFEMP1 as a Potential Biomarker for Diagnosis and Prognosis of Osteosarcoma
- Author
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Lei-Lei Huang, Huisi Ma, Dongliang Zhao, Zhong-Wei Lin, Wen-Lin Xie, Zhuo Wang, Jiayan Lian, and Jihui Kang
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0301 basic medicine ,Male ,Lung Neoplasms ,medicine.medical_treatment ,Gastroenterology ,Metastasis ,Mice ,0302 clinical medicine ,Neoplasm Metastasis ,Lung ,education.field_of_study ,Extracellular Matrix Proteins ,Mice, Inbred BALB C ,Osteosarcoma ,General Medicine ,Prognosis ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,Medicine ,Female ,Research Article ,Adult ,medicine.medical_specialty ,Article Subject ,Adolescent ,Patients ,Mice, Nude ,Malignancy ,General Biochemistry, Genetics and Molecular Biology ,Bone and Bones ,03 medical and health sciences ,Extracellular matrix protein 1 ,Young Adult ,Internal medicine ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Animals ,Humans ,education ,Chemotherapy ,General Immunology and Microbiology ,business.industry ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,Tumor progression ,Chondrosarcoma ,business - Abstract
Osteosarcoma (OS) is the most common primary bone malignancy. Our previous study revealed an association between the level of epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) and the invasion, metastasis, and poor prognosis of OS. However, the exact correlation between the serum EFEMP1 level and OS diagnosis and progression was unclear. This study is aimed at determining the value of the serum EFEMP1 level in the diagnosis and prognosis of OS. Fifty-one consecutive OS patients were prospectively registered in this study. The serum EFEMP1 levels were measured using ELISA at diagnosis, after neoadjuvant chemotherapy, and before and after surgical treatment. Sixty-nine healthy subjects in the control group, nine patients with chondrosarcoma, and 12 patients with giant cell tumor of the bone were also enrolled in this study. Surgical orthotopic implantation was used to generate a mouse OS model, and the correlation between the circulating EFEMP1 levels and tumor progression was examined. Then, OS patients had significantly higher mean serum EFEMP1 levels (7.61 ng/ml) than the control subjects (1.47 ng/ml). The serum EFEMP1 levels were correlated with the Enneking staging system (r=0.32, P=0.021) and lung metastasis (r=0.50, P<0.001). There was also a correlation between the serum EFEMP1 level and EFEMP1 expression in the respective OS samples (r=0.49, P<0.001). Additionally, patients with either chondrosarcoma or giant cell tumor of the bone had significantly higher serum EFEMP1 levels than OS patients. Surgical and chemotherapeutic treatment led to an increase in the serum EFEMP1 levels. Then, the destruction of bone tissues might be one of the factors about the EFEMP1 levels. In the mouse OS model, the serum EFEMP1 level was correlated with tumor progression. Our results suggested that serum EFEMP1 levels might be used to distinguish OS patients from healthy controls and as an indicator for OS lung metastasis. Serum EFEMP1 levels could serve as a new and assisted biomarker for the auxiliary diagnosis and prognosis of OS.
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- 2020
4. Prediction of MicroRNA and Gene Target in Synovium-Associated Pain of Knee Osteoarthritis Based on Canonical Correlation Analysis
- Author
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Wang, Haiming, primary, Hu, Yue, additional, Xie, Yujie, additional, Wang, Li, additional, Wang, Jianxiong, additional, Lei, Lei, additional, Huang, Maomao, additional, and Zhang, Chi, additional
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- 2019
- Full Text
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5. Advances in the Diagnosis and Treatment of Acute Kidney Injury in Cirrhosis Patients
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Lei, Lei, Li, Liangping, and Zhang, Hu
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Liver Cirrhosis ,medicine.medical_specialty ,Cirrhosis ,lcsh:Medicine ,Renal function ,Review Article ,urologic and male genital diseases ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Liver disease ,0302 clinical medicine ,medicine ,Humans ,Decompensation ,Stage (cooking) ,Intensive care medicine ,Creatinine ,General Immunology and Microbiology ,urogenital system ,business.industry ,Mortality rate ,Sodium ,lcsh:R ,Acute kidney injury ,Water ,030208 emergency & critical care medicine ,General Medicine ,Acute Kidney Injury ,Prognosis ,medicine.disease ,female genital diseases and pregnancy complications ,chemistry ,030211 gastroenterology & hepatology ,business ,Glomerular Filtration Rate - Abstract
Liver cirrhosis is a common progressive and chronic clinical liver disease. Due to the strong compensation ability of the liver, no obvious symptoms develop in the early stage. However, multiple systems are involved in decompensation of the liver. Acute kidney injury (AKI) is one of the most serious complications, characterized by a sharp drop in the glomerular filtration rate (GFR); a rapid increase in Scr and BUN, as well as sodium and water storage; and a disturbance of acid-base balance. The mortality rate is high, and the prognosis is very poor. Thus, it is important to make a definite diagnosis and initiate treatment in the early stage to decrease mortality and improve the prognosis. Although diagnosing liver cirrhosis with serum creatinine has many shortcomings, a dynamic change in this marker is still the main diagnostic criterion for AKI. Identifying new markers of kidney injury with clinical value has also become an increasing focus of research. In this text, we review recent changes regarding categorization of AKI diagnostic criteria as well as new markers of AKI and treatments for cirrhosis-related AKI.
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- 2017
6. Prediction of MicroRNA and Gene Target in Synovium-Associated Pain of Knee Osteoarthritis Based on Canonical Correlation Analysis
- Author
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Yu-jie Xie, Lei Lei, Chi Zhang, Jian-xiong Wang, Yue Hu, Mao-Mao Huang, Li Wang, and Haiming Wang
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0301 basic medicine ,Knee Joint ,Article Subject ,lcsh:Medicine ,Pain ,Computational biology ,Osteoarthritis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,Databases, Genetic ,medicine ,OMIM : Online Mendelian Inheritance in Man ,Humans ,Gene Regulatory Networks ,RNA, Messenger ,KEGG ,Gene ,Aged ,030203 arthritis & rheumatology ,General Immunology and Microbiology ,Receiver operating characteristic ,Gene Expression Profiling ,lcsh:R ,Synovial Membrane ,Computational Biology ,General Medicine ,Middle Aged ,Osteoarthritis, Knee ,medicine.disease ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Disease Progression ,Biomarker (medicine) ,Female ,Synovial membrane ,Biomarkers ,Research Article - Abstract
Inflammation plays a central role in knee osteoarthritis (OA) pathogenesis (C. R. Scanzello, 2017). The synovial membrane inflammation is associated with disease progression and represents a primary source of agony in knee OA (L. A. Stoppiello et al., 2014). Many inflammatory mediators may have biomarker utility. To identify synovium related to knee OA pain biomarkers, we used canonical correlation analysis to analyze the miRNA-mRNA dual expression profiling data and extracted the miRNAs and mRNAs. After identifying miRNAs and mRNAs, we built an interaction network by integrating miRWalk2.0. Then, we extended the network by increasing miRNA-mRNA pairs and identified five miRNAs and four genes (TGFBR2, DST, TBXAS1, and FHLI) through the Spearman rank correlation test. For miRNAs involved in the network, we further performed the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses, whereafter only those mRNAs overlapped with the Online Mendelian Inheritance in Man (OMIM) genetic database were analyzed. Receiver operating characteristic (ROC) curve and support vector machine (SVM) classification were taken into the analysis. The results demonstrated that all the recognized miRNAs and their gene targets in the network might be potential biomarkers for synovial-associated pain in knee OA. This study predicts the underlying risk biomarkers of synovium pain in knee OA.
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- 2019
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7. High Vimentin Expression Predicts a Poor Prognosis and Progression in Colorectal Cancer: A Study with Meta-Analysis and TCGA Database
- Author
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Du, Le, Li, Jingchuan, Lei, Lei, He, Hongjuan, Chen, Erfei, Dong, Jing, and Yang, Jin
- Subjects
Article Subject - Abstract
The aim of this study was to evaluate the role of vimentin expression in the prognosis and progression of CRC. Meta-analysis was conducted to investigate the correlations between vimentin and prognosis and clinicopathological features in CRC. Literatures were searched by PubMed, Embase, ClinicalKey, CNKI, VIP, and WanFang databases. The Cancer Genome Atlas (TCGA) database was used to assess the association of vimentin expression with survival rate in CRC. Eleven reports with 1969 cases were included in the meta-analysis. The results showed that positive vimentin expression predicted a poor overall survival (OS) in the univariate analysis (HR: 2.087, 95%CI: 1.660-2.625) and multivariate analysis (HR: 1.633, 95%CI: 1.223-2.181). Vimentin overexpression also conferred worse disease-free survival (DFS) in the univariate analysis (HR: 2.069, 95%CI: 1.024-4.179) and multivariate analysis (HR: 2.802, 95%CI: 1.421-5.527). Moreover, upregulated vimentin is related to lymph node metastasis (OR: 2.288, 95%CI: 1.159-4.517), TNM stages (OR: 1.957, 95%CI: 1.333-2.873), and N stage (OR: 2.316, 95%CI: 1.482-3.620). Analysis of TCGA database indicated that elevated vimentin predicated a shorter OS (p=0.033). Our findings reveal that upregulated vimentin contributes to the progression and poor prognosis of CRC. Vimentin may be a prognostic biomarker and therapeutic target in patients with CRC.
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- 2018
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8. High Vimentin Expression Predicts a Poor Prognosis and Progression in Colorectal Cancer: A Study with Meta-Analysis and TCGA Database
- Author
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Hongjuan He, Erfei Chen, Le Du, Jin Yang, Jing Dong, Jingchuan Li, and Lei Lei
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Male ,0301 basic medicine ,Multivariate analysis ,Colorectal cancer ,lcsh:Medicine ,Vimentin ,Review Article ,computer.software_genre ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Biomarkers, Tumor ,Humans ,Medicine ,Survival rate ,Survival analysis ,Vimentin expression ,Univariate analysis ,General Immunology and Microbiology ,Database ,biology ,business.industry ,lcsh:R ,General Medicine ,Prognosis ,medicine.disease ,Survival Analysis ,030104 developmental biology ,030220 oncology & carcinogenesis ,Meta-analysis ,Disease Progression ,biology.protein ,Female ,Colorectal Neoplasms ,business ,computer ,Biomarkers - Abstract
The aim of this study was to evaluate the role of vimentin expression in the prognosis and progression of CRC. Meta-analysis was conducted to investigate the correlations between vimentin and prognosis and clinicopathological features in CRC. Literatures were searched by PubMed, Embase, ClinicalKey, CNKI, VIP, and WanFang databases. The Cancer Genome Atlas (TCGA) database was used to assess the association of vimentin expression with survival rate in CRC. Eleven reports with 1969 cases were included in the meta-analysis. The results showed that positive vimentin expression predicted a poor overall survival (OS) in the univariate analysis (HR: 2.087, 95%CI: 1.660-2.625) and multivariate analysis (HR: 1.633, 95%CI: 1.223-2.181). Vimentin overexpression also conferred worse disease-free survival (DFS) in the univariate analysis (HR: 2.069, 95%CI: 1.024-4.179) and multivariate analysis (HR: 2.802, 95%CI: 1.421-5.527). Moreover, upregulated vimentin is related to lymph node metastasis (OR: 2.288, 95%CI: 1.159-4.517), TNM stages (OR: 1.957, 95%CI: 1.333-2.873), and N stage (OR: 2.316, 95%CI: 1.482-3.620). Analysis of TCGA database indicated that elevated vimentin predicated a shorter OS (p=0.033). Our findings reveal that upregulated vimentin contributes to the progression and poor prognosis of CRC. Vimentin may be a prognostic biomarker and therapeutic target in patients with CRC.
- Published
- 2018
9. Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy
- Author
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Sun, Rui-zhen, primary, Fan, Ying, additional, Liang, Xiao, additional, Gong, Tian-tian, additional, Wang, Qi, additional, Liu, Hui, additional, Shan, Zhi-yan, additional, and Lei, Lei, additional
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- 2018
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10. Association and Interaction Analysis of Body Mass Index and Triglycerides Level with Blood Pressure in Elderly Individuals in China
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Zhang, Lin, primary, Li, Jin-long, additional, Zhang, Li-li, additional, Guo, Lei-lei, additional, Li, Hong, additional, and Li, Dan, additional
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- 2018
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11. Intensity-dependent effect of treadmill running on knee articular cartilage in a rat model
- Author
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Yue-Zhu Zhou, Guo-Xin Ni, Lei Lei, Sheng-Yao Liu, Li-Qiong Zhan, and Zhe Li
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Cartilage, Articular ,medicine.medical_specialty ,Article Subject ,Rat model ,lcsh:Medicine ,Articular cartilage ,General Biochemistry, Genetics and Molecular Biology ,Chondrocyte ,Treadmill running ,Internal medicine ,Physical Conditioning, Animal ,medicine ,Animals ,Humans ,Knee ,computer.programming_language ,General Immunology and Microbiology ,sed ,business.industry ,Cartilage ,lcsh:R ,Significant difference ,General Medicine ,Anatomy ,Intensity (physics) ,Rats ,medicine.anatomical_structure ,Endocrinology ,Exercise Test ,business ,computer ,Research Article - Abstract
Objective. To understand the changes of femoral cartilage in response to treadmill running with different intensities in the hope of differentiating “moderate” and “strenuous” running in a rat model.Method. A total of 24 male Wistar rats were randomly assigned into groups of sedentary (SED), low-intensity running (LIR), medium-intensity running (MIR), and high-intensity running (HIR). Rats in LIR, MIR, and HIR groups underwent 8 weeks’ treadmill running programs. After sacrificed, femoral condyles were collected to take histomorphometric analysis and immunohistochemistry for collagen II.Results. Gross and histological observation showed osteoarthritic changes in group HIR. In comparison to SED group, there was significant increase in cartilage thickness, number of chondrocytes, and GAG content in groups LIR and MIR. Conversely, decrease in cartilage thickness, chondrocyte number, and GAG content was found in rats of HIR group, without significant difference though. In addition, in comparison to SED group, HIR group exhibited disorganization of collagen fibril and significantly lower content of collagen type II.Conclusion. An intensity-dependent effect was suggested on the articular cartilage. Our results also demonstrated that running with low-to-medium intensity applied in the present study should be regarded as “moderate” running, whereas high-intensity running as “strenuous” running.
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- 2013
12. Improved Early Postresuscitation EEG Activity for Animals Treated with Hypothermia Predicted 96 hr Neurological Outcome and Survival in a Rat Model of Cardiac Arrest
- Author
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Bihua Chen, Yongqin Li, Meng-Hua Chen, Wei-Ni Gan, Feng-Qing Song, Lei-Lei Sun, and Ling-Yan Lei
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Resuscitation ,Article Subject ,Entropy ,medicine.medical_treatment ,Rat model ,lcsh:Medicine ,Electroencephalography ,General Biochemistry, Genetics and Molecular Biology ,Hypothermia, Induced ,medicine ,Animals ,Humans ,Cardiopulmonary resuscitation ,General Immunology and Microbiology ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Brain ,Heart ,General Medicine ,Hypothermia ,Cardiopulmonary Resuscitation ,Rats ,Disease Models, Animal ,Burst suppression ,Eeg activity ,Anesthesia ,Heart Arrest, Induced ,Aortic pressure ,medicine.symptom ,business ,Research Article - Abstract
Purpose.To investigate the effect of hypothermia on 96 hr neurological outcome and survival by quantitatively characterizing early postresuscitation EEG in a rat model of cardiac arrest.Materials and Methods.In twenty male Sprague-Dawley rats, cardiac arrest was induced through high frequency transesophageal cardiac pacing. Cardiopulmonary resuscitation was initiated after 5 mins untreated arrest. Immediately after resuscitation, animals were randomized to either 2 hrs of hypothermia (N=10) or normothermia (N=10). EEG, ECG, aortic pressure, and core temperature were continuously recorded for 6 hrs. Neurological outcome was evaluated daily during the 96 hrs postresuscitation period.Results.No differences in the baseline measurements and resuscitation outcome were observed between groups. However, 96 hr neurological deficit score (204 ± 255 versus 500 ± 0,P=0.005) and survival (6/10 versus 0/10,P=0.011) were significantly better in the hypothermic group. Quantitative analysis of early postresuscitation EEG revealed that burst frequency and spectrum entropy were greatly improved in the hypothermic group and correlated with 96 hr neurological outcome and survival.Conclusion.The improved burst frequency during burst suppression period and preserved spectrum entropy after restoration of continuous background EEG activity for animals treated with hypothermia predicted favorable neurological outcome and survival in this rat model of cardiac arrest.
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- 2013
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13. Intensity-Dependent Effect of Treadmill Running on Knee Articular Cartilage in a Rat Model
- Author
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Ni, Guo-Xin, primary, Liu, Sheng-Yao, additional, Lei, Lei, additional, Li, Zhe, additional, Zhou, Yue-Zhu, additional, and Zhan, Li-Qiong, additional
- Published
- 2013
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14. Improved Early Postresuscitation EEG Activity for Animals Treated with Hypothermia Predicted 96 hr Neurological Outcome and Survival in a Rat Model of Cardiac Arrest
- Author
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Chen, Bihua, primary, Song, Feng-Qing, additional, Sun, Lei-Lei, additional, Lei, Ling-Yan, additional, Gan, Wei-Ni, additional, Chen, Meng-Hua, additional, and Li, Yongqin, additional
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- 2013
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15. Intensity-Dependent Effect of Treadmill Running on Knee Articular Cartilage in a Rat Model.
- Author
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Guo-Xin Ni, Sheng-Yao Liu, Lei Lei, Zhe Li, Yue-Zhu Zhou, and Li-Qiong Zhan
- Abstract
Objective. To understand the changes of femoral cartilage in response to treadmill running with different intensities in the hope of differentiating "moderate" and "strenuous" running in a rat model. Method. A total of 24 male Wistar rats were randomly assigned into groups of sedentary (SED), low-intensity running (LIR), medium-intensity running (MIR), and high-intensity running (HIR). Rats in LIR, MIR, and HIR groups underwent 8 weeks' treadmill running programs. After sacrificed, femoral condyles were collected to take histomorphometric analysis and immunohistochemistry for collagen II. Results. Gross and histological observation showed osteoarthritic changes in group HIR. In comparison to SED group, there was significant increase in cartilage thickness, number of chondrocytes, and GAG content in groups LIR and MIR. Conversely, decrease in cartilage thickness, chondrocyte number, and GAG content was found in rats of HIR group, without significant difference though. In addition, in comparison to SED group, HIR group exhibited disorganization of collagen fibril and significantly lower content of collagen type II. Conclusion. An intensity-dependent effect was suggested on the articular cartilage. Our results also demonstrated that running with low-to-medium intensity applied in the present study should be regarded as "moderate" running, whereas high-intensity running as "strenuous" running. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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