1. Expression of MiR-608 in Nonsmall Cell Lung Cancer and Molecular Mechanism of Apoptosis and Migration of A549 Cells.
- Author
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Huang, Chu, Yue, Weiming, Li, Lin, Li, Shuhai, Gao, Cun, Si, Libo, Qi, Lei, Cheng, Chuanle, Lu, Ming, and Tian, Hui
- Subjects
APOPTOSIS ,BLOOD collection ,CANCER patients ,CARRIER proteins ,CELL lines ,CELL motility ,DNA ,GENE expression ,GENETIC polymorphisms ,LUNG cancer ,POLYMERASE chain reaction ,WESTERN immunoblotting ,MICRORNA ,SEQUENCE analysis ,GENOTYPES - Abstract
Objective. This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods. Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results. MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion. The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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