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Your search keyword '"Chen, Chuan-Mu"' showing total 5 results
Start Over Author "Chen, Chuan-Mu" Journal biomed research international
5 results on '"Chen, Chuan-Mu"'

1. The Role of BPIFA1 in Upper Airway Microbial Infections and Correlated Diseases

Author

Tsou, Yung-An, Tung, Min-Che, Alexander, Katherine A., Chang, Wen-Dien, Tsai, Ming-Hsui, Chen, Hsiao-Ling, and Chen, Chuan-Mu

Subjects
Article Subject, otorhinolaryngologic diseases, respiratory system
Abstract

The mucosa is part of the first line of immune defense against pathogen exposure in humans and prevents viral and bacterial infection of the soft palate, lungs, uvula, and nasal cavity that comprise the ear-nose-throat (ENT) region. Bactericidal/permeability-increasing fold containing family A, member 1 (BPIFA1) is a secretory protein found in human upper aerodigestive tract mucosa. This innate material is secreted in mucosal fluid or found in submucosal tissue in the human soft palate, lung, uvula, and nasal cavity. BPIFA1 is a critical component of the innate immune response that prevents upper airway diseases. This review will provide a brief introduction of the roles of BPIFA1 in the upper airway (with a focus on the nasal cavity, sinus, and middle ear), specifically its history, identification, distribution in various human tissues, function, and diagnostic value in various upper airway infectious diseases.

Published
2018
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5. Methylation-associated gene silencing of RARB in areca carcinogens induced mouse oral squamous cell carcinoma.

Author

Lai ZL, Tsou YA, Fan SR, Tsai MH, Chen HL, Chang NW, Cheng JC, and Chen CM

Subjects
4-Nitroquinoline-1-oxide, Animals, Areca, Arecoline, Carcinogens, Cell Line, Tumor, CpG Islands genetics, Disease Models, Animal, Disease Progression, Humans, Male, Methyltransferases genetics, Methyltransferases metabolism, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Receptors, Retinoic Acid metabolism, Reproducibility of Results, Sequence Analysis, DNA, Carcinoma, Squamous Cell genetics, DNA Methylation genetics, Gene Silencing, Mouth Neoplasms genetics, Receptors, Retinoic Acid genetics
Abstract

Regarding oral squamous cell carcinoma (OSCC) development, chewing areca is known to be a strong risk factor in many Asian cultures. Therefore, we established an OSCC induced mouse model by 4-nitroquinoline-1-oxide (4-NQO), or arecoline, or both treatments, respectively. These are the main two components of the areca nut that could increase the occurrence of OSCC. We examined the effects with the noncommercial MCGI (mouse CpG islands) microarray for genome-wide screening the DNA methylation aberrant in induced OSCC mice. The microarray results showed 34 hypermethylated genes in 4-NQO plus arecoline induced OSCC mice tongue tissues. The examinations also used methylation-specific polymerase chain reaction (MS-PCR) and bisulfite sequencing to realize the methylation pattern in collected mouse tongue tissues and human OSCC cell lines of different grades, respectively. These results showed that retinoic acid receptor β (RARB) was indicated in hypermethylation at the promoter region and the loss of expression during cancer development. According to the results of real-time PCR, it was shown that de novo DNA methyltransferases were involved in gene epigenetic alternations of OSCC. Collectively, our results showed that RARB hypermethylation was involved in the areca-associated oral carcinogenesis.

Published
2014
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