1. Luteolin Prevents H2O2-Induced Apoptosis in H9C2 Cells through Modulating Akt-P53/Mdm2 Signaling Pathway
- Author
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Qiyan Wang, Linghui Lu, Yong Wang, Chun Li, Hong Chang, Qian Zhang, Kuiyuan Huo, and Wei Wang
- Subjects
0301 basic medicine ,Article Subject ,Cell Survival ,Cell ,lcsh:Medicine ,Apoptosis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,MTT assay ,Viability assay ,RNA, Messenger ,Luteolin ,Protein kinase B ,Cell Shape ,General Immunology and Microbiology ,lcsh:R ,Proto-Oncogene Proteins c-mdm2 ,General Medicine ,Hydrogen Peroxide ,Molecular biology ,Cell biology ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Gene Expression Regulation ,Cytoprotection ,Apoptotic signaling pathway ,Quercetin ,Signal transduction ,Tumor Suppressor Protein p53 ,Proto-Oncogene Proteins c-akt ,Research Article ,Signal Transduction - Abstract
Introduction.Luteolin, a falconoid compound in many Chinese herbs and formula, plays important roles in cardiovascular diseases. The underlying mechanism of luteolin remains to be further elaborated.Methods. A model of hydrogen peroxide- (H2O2-) induced H9C2 cells apoptosis was established. Cell viabilities were examined with an MTT assay.2′,7′-Dichlorofluorescin diacetate (DCFH-DA) and flow cytometry were used to detect ROS level and apoptosis rate, respectively. The expressions of signaling proteins related to apoptosis were analyzed by western blot and mRNA levels were detected by real-time polymerase chain reaction (PCR). Quercetin was applied as positive drug.Results. Incubation with various concentrations of H2O2(0, 50, 100, and 200 μM) for 1 h caused dose-dependent loss of cell viability and 100 μM H2O2reduced the cell viability to approximately 50%. Treatments with luteolin and quercetin protected cells from H2O2-induced cytotoxicity and reduced cellular ROS level and apoptosis rate. Moreover, luteolin could downregulate the expressions of Bax, caspase-8, cleaved-caspase-3, and p53 in apoptotic signaling pathway. Further study showed that the expressions of Akt, Bcl-2, and Mdm2 were upregulated by luteolin.Conclusion. Luteolin protects H9C2 cells from H2O2-induced apoptosis. The protective and antiapoptotic effects of luteolin could be mediated by regulating the Akt-P53/Mdm2 apoptotic pathway.
- Published
- 2016