1. Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method
- Author
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Paulo Eduardo Brandão, G. Comenale, J. Mazzuchelli-de-Souza, S. L. M. R. Assaf, Thatiana Correa de Melo, Cláudia Rodrigues Pozzi, Enio Mori, Leonardo José Richtzenhain, Mariana Santos de Miranda, R.C. Stocco, Juliana Rodrigues Pozzi Arcaro, Sônia Tatsumi Sakata, R.F. Carvalho, D.N.S. Giovanni, Willy Beçak, Secretaria Estado Saude São Paulo, Universidade de São Paulo (USP), CAPTA Bovinos Leiteiros, Universidade Federal de São Paulo (UNIFESP), and Univ Fed,Integracao Latino Americana UNILA
- Subjects
Article Subject ,Biopsy ,viruses ,Cattle Diseases ,lcsh:Medicine ,Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,DNA sequencing ,law.invention ,law ,Polymorphism (computer science) ,medicine ,Animals ,Gene ,Polymerase chain reaction ,Phylogeny ,Bovine papillomavirus ,General Immunology and Microbiology ,biology ,Coinfection ,Papillomavirus Infections ,lcsh:R ,Nucleic acid sequence ,General Medicine ,biology.organism_classification ,medicine.disease ,Virology ,Deltapapillomavirus ,Cattle ,SEQUENCIAMENTO GENÉTICO ,Restriction fragment length polymorphism ,Warts ,Brazil ,Polymorphism, Restriction Fragment Length ,Research Article - Abstract
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação do Desenvolvimento Administrativo (PAP-FUNDAP) Bovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types of BPV are currently characterized and classified into three distinct genera, associated with different pathological outcomes. the described BPV types as well as other putative ones have been demonstrated by molecular biology methods, mainly by the employment of degenerated PCR primers. Specifically, divergences in the nucleotide sequence of the L1 gene are useful for the identification and classification of new papillomavirus types. On the present work, a method based on the PCR-RFLP technique and DNA sequencing was evaluated as a screening tool, allowing for the detection of two relatively rare types of BPV in lesions samples from a six-year-old Holstein dairy cow, chronically affected with cutaneous papillomatosis. These findings point to the dissemination of BPVs with unclear pathogenic potential, since two relatively rare, new described BPV types, which were first characterized in Japan, were also detected in Brazil. Secretaria Estado Saude São Paulo, Inst Butantan, Genet Lab, BR-05503900 São Paulo, Brazil Univ São Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Lab Biol Mol Aplicada & Sorol, BR-05508270 São Paulo, Brazil CAPTA Bovinos Leiteiros, Agencia Paulista Tecnol Agronegocios, Inst Zootecnia, Ctr Pesquisa Pecuaria Leite, BR-13460000 Nova Odessa, SP, Brazil Univ São Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 São Paulo, Brazil Universidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil Univ Fed,Integracao Latino Americana UNILA, Dept Biol, BR-85867970 Foz de Iguacu, PR, Brazil Universidade Federal de São Paulo, Programa Posgrad Biol Estrutural & Func, BR-04023900 São Paulo, Brazil CNPq: 402539/2011-7 Web of Science
- Published
- 2013