1. Distribution of Microbiota in Fine Particulate Matter Particles in Guangzhou, China.
- Author
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Dong SR, Han YJ, Wu J, Zeng CL, Zhu KH, Chen XJ, Liu YM, Zou XQ, Zheng SL, Wen ZH, Liu DD, Wang Y, Huang XX, Du XB, Hao JL, Wang HY, Guo S, Jing CX, and Yang G
- Subjects
- Bacteria classification, China, Cities, Environmental Monitoring, Particle Size, RNA, Bacterial analysis, RNA, Ribosomal, 16S analysis, Air Microbiology, Air Pollutants analysis, Bacteria isolation & purification, Microbiota, Particulate Matter analysis
- Abstract
Objective: High PM
2.5 concentration is the main feature of increasing haze in developing states, but information on its microbial composition remains very limited. This study aimed to determine the composition of microbiota in PM2.5 in Guangzhou, a city located in the tropics in China., Methods: In Guangzhou, from March 5th to 10th , 2016, PM2.5 was collected in middle volume air samplers for 23 h daily. The 16S rDNA V4 region of the PM2.5 sample extracted DNA was investigated using high-throughput sequence., Results: Among the Guangzhou samples, Proteobacteria , Bacteroidetes , Firmicutes , Cyanobacteria , and Actinobacteria were the dominant microbiota accounting for more than 90% of the total microbiota, and Stenotrophomonas was the dominant gram-negative bacteria, accounting for 21.30%-23.57%. We examined the difference in bacterial distribution of PM2.5 between Beijing and Guangzhou at the genus level; Stenotrophomonas was found in both studies, but Escherichia was only detected in Guangzhou., Conclusion: In conclusion, the diversity and specificity of microbial components in Guangzhou PM2.5 were studied, which may provide a basis for future pathogenicity research in the tropics., (Copyright © 2020 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)- Published
- 2020
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