1. A novel approach to the quantification of urinary aryl‐propionamide‐derived SARMs by UHPLC–MS/MS
- Author
-
A. A. Azaryan, E. V. Dmitrieva, E. M. Gashimova, and A. Z. Temerdashev
- Subjects
Analyte ,Coefficient of determination ,Calibration curve ,Clinical Biochemistry ,Urine ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Limit of Detection ,Tandem Mass Spectrometry ,Drug Discovery ,Androgen Receptor Antagonists ,Humans ,Anilides ,Molecular Biology ,Chromatography, High Pressure Liquid ,Pharmacology ,Detection limit ,Chloroform ,Chromatography ,Extraction (chemistry) ,Reproducibility of Results ,General Medicine ,chemistry ,Androgens ,Linear Models ,Enobosarm - Abstract
A simple and sensitive procedure for the quantification of two commonly abused aryl-propionamide-derived selective androgen receptor modulators (SARMs), namely S-4 (GTx-007, andarine) and S-22 (GTx-024, MK-2866, ostarine, enobosarm), has been described. Urine samples were prepared for analysis by means of a dispersive liquid-liquid microextraction using methanol and chloroform as dispersive and extracting solvents, respectively. Factors that might influence the extraction process as well as their optimum conditions were evaluated by Box-Benken and central composite designs. After extraction, the analytes were quantified by UHPLC-MS/MS. The proposed procedure was validated on human urine samples. As a result, for both SARMs the detection limits were observed at 0.05 ng/mL and calibration curves were linear in the concentration range of 0.25-50 ng/mL with the coefficient of determination of 0.998.
- Published
- 2019