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We demonstrate model-based image reconstruction of Raman measurements obtained experimentally. Results show that Raman characterization with accurate localization is possible with anatomical priors. This method has been extended to Raman imaging of dog tibia in-vivo.

3D MRg-NIRS imaging of breast cancer has been studied in simulation and in vivo using parallel plate and circular geometries. Optimal methods to minimize errors in measurement and modeling are presented.

Protoporphyrin IX fluorescence activity in mouse model gliomas is imaged in nude mice using an MRI-coupled spectroscopy scanner. Segmented MR images acquired simultaneously with fluorescence spectra are used to guide fluorescence yield reconstruction.

Spectrally-resolved BioLuminescence Tomography is used to image Luciferase activity within a 3-D model. The Adjoint theorem for 3-D image reconstruction is presented and validated using experimental data with a complete computation time of 5 minutes.

Fast frame rate frequency domain tomography of normal breast tissue showed correlation between applied pressure and total hemoglobin, water and scattering amplitude, but no significant changes in tissue oxygen saturation and scattering power

The dynamic processes of forced hyperoxia and hypoxia in the plantar surface of the foot were characterized by a combined approach using NIR spectroscopy and electron paramagnetic resonance with great reproducibility.

A Ti:Sapphire laser based frequency-domain NIR tomography system was created with custom heterodyne detection. Gelatin phantom imaging with varying numbers of wavelengths showed superior recovery of hemoglobin concentration with increasing wavelengths included.

Using a Jacobian reconstruction method using experimental data, we show a dramatic increase in image reconstruction time in 3-D DOT, without detriment to image quality.

A method is developed to show that instead of using data from the entire spectrum, only selected spectral bands are required to improve image reconstruction accuracy in spectral diffuse optical tomography.

A NIR diffuse tomography system with spectrally-encoded sources allows simultaneous detection of all data. It can provide images of high-contrast, fast changes in tissue optical properties to be overlayed on the magnetic resonance breast scan. Article not available.

Diffuse optical fluorescence tomography often relies on the assumption that samples are homogeneous. This degrades the correspondence between tomography data sets and model predictions. We provide evidence that data normalization significantly improve on this situation. Article not available.

Singlet oxygen is believed to be the species destroying cancerous cells during PDT. An optically-based imaging device for monitoring singlet oxygen production in a tumor was developed and tested in vivo.

Spectrally-constrained diffuse tomography is used to image endogenous and exogenous tissue chromophore concentrations with higher accuracy than when using individual wavelengths. Reconstructed images from simulated data demonstrate that concentrations of Lutetium Texaphyrin are readily recovered.

Aminolevulinic acid induced Protoporphyrin IX fluorescence accumulates preferentially in brain tumor tissue. Microscopic and macroscopic studies are contrasted to determine the utility as a diagnostic measure of tumor extent in vivo for tracking therapeutic response.

The algorithms for near-infrared (NIR) diffuse optical tomography within magnetic resonance imaging (MRI) of the female breast are analyzed in both circular and curved slab geometries.

A novel spread-spectral-encoding technique that utilizes single broadband light source to achieve video-rate near-infrared diffuse optical tomography is demonstrated. This approach presents the potential of implementing near-infrared diffuse optical tomography at catheter-scale.

Spectrally-resolved BioLuminescence Tomography is used to image Luciferase activity within a 3D mouse model using multi-wavelength data from multiple bioluminescence sources. Images show the sources reconstructed accurately using a fast, direct and linear 3D algorithm.

A broadband 650-950nm transmission acquisition is implemented in conjunction with a frequency-domain imaging system. For breast tissues and phantoms, most spectra can be obtained at all source-detection positions with an exposure time less than 40min.

A fluorescence diffuse optical tomography system capable of producing B-scan-type images of localized fluorescence regions is presented. The B-Scan mode is analogous to ultrasound where the excitation and remission signals are delivered from the tissue surface.

The Mie-type scattering parameter (average particle size) can be estimated from the angular-scattering measurements taken in a goniometer system. Breast tissue samples were characterized in this manner, to estimate average particle size.

A novel scheme for implementing spectral constraints into a three-dimensional image reconstruction model is presented. This approach, based on selecting measurements relating to spatial areas of maximum heterogeneity, makes spectral imaging computationally efficient in 3-D.