1. Identification of rhodamine 123-positive stem cell subpopulations in canine hepatocellular carcinoma cells
- Author
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Yoshiki Itoh, Harumichi Itoh, Yasuho Taura, Munekazu Nakaichi, Shimpei Nishikawa, Toshie Iseri, Kenji Tani, Yu Arikawa, Kazuhito Itamoto, Tomoya Haraguchi, and Masato Hiyama
- Subjects
0301 basic medicine ,Homeobox protein NANOG ,Cluster of differentiation ,General Neuroscience ,Canine Hepatocellular Carcinoma ,General Medicine ,Articles ,Cell cycle ,Biology ,Rhodamine 123 ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,chemistry ,Cancer stem cell ,030220 oncology & carcinogenesis ,Cancer research ,General Pharmacology, Toxicology and Pharmaceutics ,Stem cell ,A431 cells - Abstract
The majority of cases of chemotherapy for hepatocellular carcinoma (HCC) are not effective in human or veterinary medicine due to resistance against anticancer agents. In human medicine, hepatocellular carcinoma stem cells (HCSCs) were recently identified as cytokeratin 19 (CK19)-, cluster of differentiation (CD)-44-, and CD133-positive. However, there are few previous reports regarding canine HCSC (cHCSC). Additionally, to the best of our knowledge, the chemoresistance against anticancer agents of these cHCSCs has not been investigated. In the present study staining of cHCSCs was performed with rhodamine 123, a low-toxicity fluorescent dye for mitochondria, by flow cytometry. There were two subpopulations in the HCC cell line defined by their higher (RhoHi) and lower (RhoLo) fluorescence intensity of rhodamine 123. The RhoHi subpopulation demonstrated a higher Nanog gene expression, sphere-forming ability, and chemoresistance against gemcitabine. However, there was no significant difference between RhoHi and RhoLo regarding the proliferation rate and chemoresistance against mitoxantrone and doxorubicin. The present results indicate that the expression of rhodamine 123 identifies different stem cell subpopulations in a canine HCC cell line.
- Published
- 2017