1. Anti-inflammatory effects of glycine thymosin β4 eye drops in experimental dry eye
- Author
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Lan Li, Che Dong Yang, Ying Li, Dong Hwan Kim, Rujun Jin, Han Sun Son, Jung Han Choi, Kyung Chul Yoon, and Hyeon Jeong Yoon
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Conjunctiva ,medicine.drug_class ,medicine.medical_treatment ,Balanced salt solution ,Inflammation ,General Biochemistry, Genetics and Molecular Biology ,Anti-inflammatory ,03 medical and health sciences ,0302 clinical medicine ,Ophthalmology ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,TUNEL assay ,ocular surface ,integumentary system ,business.industry ,General Neuroscience ,Interleukin ,General Medicine ,Articles ,eye diseases ,glycine thymosin β4 ,experimental dry eye ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,inflammation ,030220 oncology & carcinogenesis ,Tumor necrosis factor alpha ,sense organs ,medicine.symptom ,business - Abstract
The aim of the present study was to investigate the anti-inflammatory effects of glycine thymosin β4 (Gly-Tβ4) eye drops, and to compare the efficacy of topical Gly-Tβ4 with Cyclosporine A (CsA) in a mouse model of experimental dry eye (EDE). Eye drops consisting of balanced salt solution (BSS), 0.1% Gly-Tβ4 or 0.05% CsA were used for treatment of EDE. Tear volume, tear film break-up time and corneal staining scores were measured after 7 and 14 days. Periodic acid-Schiff staining for conjunctival gobleT cells, TUNEL assay for corneal apoptotic positive cells, multiplex immunobead assay for interleukin (IL)-1β, IL-6, tumor necrosis factor-α and interferon-γ levels, and flow cytometry for CD4+/CCR5+ T cells were performed after 14 days. All clinical parameters showed improvement in the Gly-Tβ4 and CsA groups (all P
- Published
- 2019