1. The cytotoxicity effects of a novel Cu complex on MCF-7 human breast cancerous cells
- Author
-
Fatemeh Mohammadizadeh, Alireza Khoshdel, Azadeh Rezaei, Soudeh Khanamani Falahati-Pour, Mohammad Reza Hajizadeh, Mohammad Ali Fahmidehkar, Maryam Mohamadi, Mohammad Reza Mirzaei, and Mehdi Mahmoodi
- Subjects
0301 basic medicine ,Necrosis ,Cell Survival ,Apoptosis ,Breast Neoplasms ,General Biochemistry, Genetics and Molecular Biology ,Flow cytometry ,Biomaterials ,Inhibitory Concentration 50 ,03 medical and health sciences ,0302 clinical medicine ,Coordination Complexes ,medicine ,Humans ,Cytotoxic T cell ,MTT assay ,Cytotoxicity ,Cell Proliferation ,medicine.diagnostic_test ,Chemistry ,Metals and Alloys ,Flow Cytometry ,Molecular biology ,030104 developmental biology ,MCF-7 ,030220 oncology & carcinogenesis ,Cancer cell ,MCF-7 Cells ,Female ,medicine.symptom ,General Agricultural and Biological Sciences ,Copper ,Phenanthrolines - Abstract
A variety of biological activities, such as anti-microbial and anti-tumor properties was reported for 1,10-phenanthroline and its copper complexes. In this study, the anti-proliferative activity of a novel [Cu(L)(phen)] complex was investigated on MCF-7 breast cancer cells using MTT assay. Since chemotherapy is lake of ability to distinguish between normal cells from cancerous cells, therefore we also investigated the effect of [Cu(L)(phen)] complex on normal L929 cells. The results showed that following 24 and 48 h exposure of cells with [Cu(L)(phen)] complex, the IC50 values for MCF-7 were significantly lower than that recorded for L929 and normal cells were less sensitive than cancerous cells to the complex. Additionally, the [Cu(L)(phen)] complex displayed a time- and concentration-dependent cytotoxic response, with MCF-7 and L929 cells. Also flow cytometry findings suggest that [Cu(L)(phen)] complex is capable of decreasing cancer cell viability through apoptosis and did not efficiently activate the necrosis process.
- Published
- 2018
- Full Text
- View/download PDF