1. Cremophor EL Nano-Emulsion Monomerizes Chlorophyll a in Water Medium.
- Author
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Janik-Zabrotowicz E, Arczewska M, Zubik M, Terpilowski K, Skrzypek TH, Swietlicka I, and Gagos M
- Subjects
- Buffers, Chlorophyll A isolation & purification, Emulsions chemistry, Ethanol chemistry, Glycerol chemistry, Phosphates chemistry, Chlorophyll A chemistry, Glycerol analogs & derivatives, Nanoparticles chemistry, Surface-Active Agents chemistry, Water chemistry
- Abstract
In this paper, the application of a non-ionic detergent Cremophor EL for monomerization of chlorophyll a in an aqueous medium is studied. The spectrophotometric properties of chlorophyll a encapsulated into the Cremophor EL nano-emulsion system were characterized by electronic absorption, steady-state and time-resolved fluorescence as well as circular dichroism spectroscopy. The results have shown that chlorophyll a dissolves more efficiently in the aqueous medium containing low-level Cremophor (5 wt%) than at an ethanolic solution even in the concentration of 10
-4 M. The molecular organization of the chlorophyll a in the Cremophor EL nano-micelles was also investigated by means of Raman spectroscopy. The spectral changes in the frequency of the C=O stretching group were used to distinguish the aggregation state of chlorophyll. It was revealed that chlorophyll a exists dominantly in the monomeric form in the Cremophor EL aqueous solution. The promising aspect of the use of Cremophor EL nano-emulsion as a delivery system is to maintain stable chlorophyll monomer in an aqueous medium. It would open the potential for a new, practical application of chlorophyll a in medicine, as a dietary supplement or studies on molecular organization of chlorophyll a in the well-defined artificial system.- Published
- 2019
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